Document Detail


Phosphanilic acid inhibits dihydropteroate synthase.
MedLine Citation:
PMID:  2514624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intact cells of Pseudomonas aeruginosa were more susceptible to phosphanilic acid (PA) than cells of Escherichia coli. In cell extracts, the dihydropteroate synthases of P. aeruginosa and E. coli were about equally susceptible to inhibition by PA. These results suggest that cells of P. aeruginosa are more permeable to PA than cells of E. coli. Although a weak inhibitor, PA acted on dihydropteroate synthase in the same manner as the sulfonamides with which PA is structurally related. Inhibition of E. coli by PA in a basal salts-glucose medium was prevented by p-aminobenzoic acid (pABA). However, pABA did not protect P. aeruginosa from PA under these conditions, possibly because pABA itself exhibited an inhibitory effect. PA also appeared to have a second mode of action. The mechanism was not elucidated.
Authors:
R G Eagon; A T McManus
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  33     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1936-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Georgia, Athens 30602.
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MeSH Terms
Descriptor/Qualifier:
4-Aminobenzoic Acid / pharmacology
Aniline Compounds / pharmacology*
Dihydropteroate Synthase / antagonists & inhibitors*
Escherichia coli / drug effects,  enzymology
Indicators and Reagents
Microbial Sensitivity Tests
Pseudomonas aeruginosa / drug effects,  enzymology
Sulfanilamides / pharmacology
Transferases / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/Aniline Compounds; 0/Indicators and Reagents; 0/Sulfanilamides; 150-13-0/4-Aminobenzoic Acid; 5337-17-7/phosphanilic acid; 63-74-1/sulfanilamide; EC 2.-/Transferases; EC 2.5.1.15/Dihydropteroate Synthase
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