| Phorbol myristate acetate stimulates pinocytosis and membrane spreading in mouse peritoneal macrophages. | |
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MedLine Citation:
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PMID: 7400219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phorbol myristate acetate (PMA) at a concentration of 0.01 microgram/ml causes an approximately threefold increase in surface area of resident, proteose-peptone-elicited, and thioglycolate-broth-elicited mouse peritoneal macrophages. Resident and proteose-peptone-elicited macrophages, cultured for 24 h in the presence of PMA, increase their pinocytic rate twofold in response to addition of PMA (0.01 microgram/ml) to the medium. Thioglycolate-broth-elicited macrophages, cultured for 24 h in the absence of PMA, immediately increase their pinocytic rate 2- to 3.5-fold in response to a single challenge with PMA (0.01 microgram/ml). Cytochalasin B, colchicine, and podophyllotoxin have only modest inhibitory effects on the basal rate of pinocytosis and on PMA-induced cellular spreading, but completely block the stimulatory effects of PMA on pinocytosis in thioglycolate-broth-elicited macrophages. Cytochalasin D markedly inhibits both basal and PMA-stimulated pinocytosis in these cells. Thus, PMA is a useful tool for studying mechanisms of macrophage spreading and for enhancing the overall rate of pinosome formation. |
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Authors:
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L Phaire-Washington; E Wang; S C Silverstein |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 86 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1980 Aug |
Date Detail:
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Created Date: 1980-10-27 Completed Date: 1980-10-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 634-40 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascitic Fluid / cytology Cell Movement / drug effects Colchicine / pharmacology Cytochalasins / pharmacology Endocytosis Horseradish Peroxidase / metabolism Macrophages / drug effects*, physiology Mice Phorbols / pharmacology* Pinocytosis / drug effects* Podophyllotoxin / pharmacology Stimulation, Chemical Tetradecanoylphorbol Acetate / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Cytochalasins; 0/Phorbols; 16561-29-8/Tetradecanoylphorbol Acetate; 518-28-5/Podophyllotoxin; 64-86-8/Colchicine; EC 1.11.1.-/Horseradish Peroxidase |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 8 Year: 1980 Volume: 86 Issue: 2 First Page: 634 Last Page: 640 ID: 2111483 Publisher Id: 80249676 PubMed Id: 7400219 |
| Phorbol myristate acetate stimulates pinocytosis and membrane spreading in mouse peritoneal macrophages | |
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