Document Detail


Phorbol myristate acetate stimulates degradation of a structural analogue of platelet-activating factor to a neutral lipid in human leukemic K562 cells: relevance to the release of lipids.
MedLine Citation:
PMID:  14709893     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In our attempt to investigate the mechanism of the release of platelet-activating factor (PAF) from cells, the erythroleukemic cell line K562 was preloaded with a radiolabeled PAF analogue having an ethylcarbamyl residue, 1-O-octadecyl-2-O-ethylcarbamyl-sn-glycero-3-phosphocholine (ethylcarbamyl-PAF), that is resistant to the hydrolytic action of PAF acetylhydrolase. Its extracellular release was monitored using an albumin back-extraction method, and its metabolic degradation was analyzed by TLC. Phorbol myristate acetate (PMA) was found to stimulate the release of two radioactive lipids, ethylcarbamyl-PAF itself and its metabolite, 1-O-octadecyl-2-ethylcarbamyl-sn-glycerol, whereas only ethylcarbamyl-PAF was released from the resting cells. The increased release of radioactive lipids in PMA-stimulated cells was suggested to be due to stimulated degradation of intracellular ethylcarbamyl-PAF into the cell-permeable metabolite. Thus K562 cells have much less capacity to release intact PAF-like lipid in comparison with its high ability to uptake exogenously added PAF analogues previously described by us and others.
Authors:
Toshihiko Tsutsumi; Akira Tokumura; Masaya Yamaguchi; Shikifumi Kitazawa; Yusuke Tanigawara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  27     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-07     Completed Date:  2004-08-10     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  24-8     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan. t-tsutsumi@phoenix.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Biotransformation
Bridged Compounds / pharmacology
Chromatography, Thin Layer
Culture Media
Humans
K562 Cells
Lipid Metabolism
Lipids / chemistry*
Platelet Activating Factor / analogs & derivatives*,  chemistry*
Sodium Fluoride / pharmacology
Stimulation, Chemical
Tetradecanoylphorbol Acetate / pharmacology*
Thiones / pharmacology
Chemical
Reg. No./Substance:
0/Bridged Compounds; 0/Culture Media; 0/Lipids; 0/O-deacetyl platelet activating factor; 0/Platelet Activating Factor; 0/Thiones; 16561-29-8/Tetradecanoylphorbol Acetate; 7681-49-4/Sodium Fluoride; 83373-60-8/tricyclodecane-9-yl-xanthogenate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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