Document Detail


Phorbol esters differentially regulate the expression of insulin-like growth factor-binding proteins in endometrial carcinoma cells.
MedLine Citation:
PMID:  1280205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the effects of protein kinase-C (PKC) activation on expression of the six known insulin-like growth factor-binding proteins (IGFBPs) by human endometrial carcinoma cells. Each of six known IGFBPs was expressed in one or more of the three cell lines examined. The addition of 10(-7) M 4 beta-phorbol 12-myristate 13-acetate (PMA) to HEC-50 and HEC-1B cells resulted in changes in cell morphology, growth inhibition, activation of PKC, and an increase in expression of IGFBP-1. PMA had no effect on these parameters in the Ishikawa cell line, which did not express IGFBP-1. In HEC-50 cells, the effect of PMA was blocked by the concomitant addition of the PKC inhibitor staurosporin and the simultaneous addition of cycloheximide. PMA also resulted in an increase in IGFBP-3 in HEC-50 cells and an increase in IGFBP-6 expression in HEC-1B cells. In contrast, IGFBP-3 expression was down-regulated by PMA in HEC-1B and Ishikawa cells. The abundance of IGFBP-2 and IGFBP-5 mRNAs was also reduced in HEC-1B and Ishikawa cells, respectively. IGFBP-4 was expressed only in HEC-50 cells and was not affected by PMA treatment. These data establish a role for the PKC pathway in regulation of expression of IGFBP-1, -2, -3, and -5 in endometrial adenocarcinoma cells and illustrate the complexity of cell type-specific expression of the IGFBPs.
Authors:
Y Gong; G Ballejo; B Alkhalaf; P Molnar; L C Murphy; L J Murphy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  131     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1992-12-30     Completed Date:  1992-12-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2747-54     Citation Subset:  AIM; IM    
Affiliation:
Department of Internal Medicine, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism*,  pathology
Alkaloids / pharmacology
Blotting, Northern
Calcimycin / pharmacology
Carrier Proteins / genetics*
Cell Division / drug effects
Cycloheximide / pharmacology
Endometrial Neoplasms / metabolism*,  pathology
Enzyme Activation
Female
Gene Expression Regulation / drug effects*
Humans
Insulin-Like Growth Factor Binding Proteins
Kinetics
Protein Kinase C / antagonists & inhibitors,  metabolism
RNA, Messenger / metabolism
Staurosporine
Terpenes / pharmacology
Tetradecanoylphorbol Acetate / pharmacology*
Thapsigargin
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Carrier Proteins; 0/Insulin-Like Growth Factor Binding Proteins; 0/RNA, Messenger; 0/Terpenes; 16561-29-8/Tetradecanoylphorbol Acetate; 52665-69-7/Calcimycin; 62996-74-1/Staurosporine; 66-81-9/Cycloheximide; 67526-95-8/Thapsigargin; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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