| Phorbol esters alter cell fate during development of sea urchin embryos. | |
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MedLine Citation:
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PMID: 1469053 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protein kinase C (PKC) has been implicated as important in controlling cell differentiation during embryonic development. We have examined the ability of 12-O-tetradecanoyl phorbol-13-acetate (TPA), an activator of PKC, to alter the differentiation of cells during sea urchin development. Addition of TPA to embryos for 10-15 min during early cleavage caused dramatic changes in their development during gastrulation. Using tissue-specific antibodies, we have shown that TPA causes the number of cells that differentiate as endoderm and mesoderm to increase relative to the number that differentiate as ectoderm. cDNA probes show that treatment with TPA causes an increase in accumulation of RNAs specific to endoderm and mesoderm with a concomitant decrease in RNAs specific to ectoderm. Treatment of isolated prospective ectodermal cells with TPA causes them to differentiate into endoderm and mesoderm. The critical period for TPA to alter development is during early to mid cleavage, and treatment of embryos with TPA after that time has little effect. These results indicate that PKC may play a key role in determining the fate of cells during sea urchin development. |
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Authors:
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B T Livingston; F H Wilt |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 119 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-01-27 Completed Date: 1993-01-27 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1641-8 Citation Subset: IM; S |
Affiliation:
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School of Biological Sciences, University of Missouri, Kansas City 64110-2499. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastomeres / physiology Cell Differentiation / drug effects* Cell Division / drug effects Cell Polarity / physiology Embryo, Nonmammalian / drug effects Endoderm / physiology Gene Expression Lithium / pharmacology Mesoderm / physiology Protein Kinase C / drug effects RNA, Messenger / analysis Sea Urchins / drug effects, embryology*, enzymology Tetradecanoylphorbol Acetate / pharmacology* Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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HD-07299/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 7439-93-2/Lithium; EC 2.7.11.13/Protein Kinase C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol Journal ID (publisher-id): J. Cell Biol. ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 12 Year: 1992 Volume: 119 Issue: 6 First Page: 1641 Last Page: 1648 ID: 2289755 Publisher Id: 93107163 PubMed Id: 1469053 |
| Phorbol esters alter cell fate during development of sea urchin embryos | |
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