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Phorbol esters alter cell fate during development of sea urchin embryos.
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MedLine Citation:
PMID:  1469053     Owner:  NLM     Status:  MEDLINE    
Protein kinase C (PKC) has been implicated as important in controlling cell differentiation during embryonic development. We have examined the ability of 12-O-tetradecanoyl phorbol-13-acetate (TPA), an activator of PKC, to alter the differentiation of cells during sea urchin development. Addition of TPA to embryos for 10-15 min during early cleavage caused dramatic changes in their development during gastrulation. Using tissue-specific antibodies, we have shown that TPA causes the number of cells that differentiate as endoderm and mesoderm to increase relative to the number that differentiate as ectoderm. cDNA probes show that treatment with TPA causes an increase in accumulation of RNAs specific to endoderm and mesoderm with a concomitant decrease in RNAs specific to ectoderm. Treatment of isolated prospective ectodermal cells with TPA causes them to differentiate into endoderm and mesoderm. The critical period for TPA to alter development is during early to mid cleavage, and treatment of embryos with TPA after that time has little effect. These results indicate that PKC may play a key role in determining the fate of cells during sea urchin development.
B T Livingston; F H Wilt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  119     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-27     Completed Date:  1993-01-27     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1641-8     Citation Subset:  IM; S    
School of Biological Sciences, University of Missouri, Kansas City 64110-2499.
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MeSH Terms
Blastomeres / physiology
Cell Differentiation / drug effects*
Cell Division / drug effects
Cell Polarity / physiology
Embryo, Nonmammalian / drug effects
Endoderm / physiology
Gene Expression
Lithium / pharmacology
Mesoderm / physiology
Protein Kinase C / drug effects
RNA, Messenger / analysis
Sea Urchins / drug effects,  embryology*,  enzymology
Tetradecanoylphorbol Acetate / pharmacology*
Tissue Distribution
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 7439-93-2/Lithium; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 12 Year: 1992
Volume: 119 Issue: 6
First Page: 1641 Last Page: 1648
ID: 2289755
Publisher Id: 93107163
PubMed Id: 1469053

Phorbol esters alter cell fate during development of sea urchin embryos

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