Document Detail

Phorbol ester pretreatment attenuates the growth hormone (GH) response to GH-releasing factor in cultured rat pituitary cells.
MedLine Citation:
PMID:  3141549     Owner:  NLM     Status:  MEDLINE    
The effect of phorbol ester pretreatment on rat (r) GH release induced by GH-releasing factor (GRF) or 8-bromo-cyclic (c)AMP was investigated using rat pituitary cells cultured in monolayers. Pretreatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 3 h significantly suppressed the rGH release induced by GRF, but not that by 8-bromo-cAMP 20 h later; this suppressive effect of TPA was concentration-dependent from 8 to 160 nmol/l, and complete suppression was observed after pretreatment with 80-160 nmol TPA/l. Production of cAMP by pituitary cells stimulated with GRF was similarly attenuated in TPA-pretreated cells. The rGH responsiveness to GRF of these cells was fully recovered on prolonged culture (40 h), suggesting that the inhibitory effect of TPA is reversible. In contrast, pretreatment with GRF (5 nmol/l) resulted in suppression of the rGH response to subsequent exposure to GRF (5 nmol/l) or 8-bromo-cAMP (10 mmol/l), but not to TPA. These observations suggest that pretreatment with TPA modifies the rGH response to GRF at steps before the formation of cAMP.
E Ohmura; M Okada; Y Ohba; N Onoda; T Sano; T Tsushima; K Shizume
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  118     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-12-22     Completed Date:  1988-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  423-8     Citation Subset:  IM    
Department of Medicine, Tokyo Women's Medical College, Japan.
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Cells, Cultured
Depression, Chemical
Growth Hormone / metabolism*
Growth Hormone-Releasing Hormone / pharmacology*
Pituitary Gland, Anterior / drug effects*,  metabolism
Rats, Inbred Strains
Tetradecanoylphorbol Acetate / pharmacology*
Time Factors
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 9002-72-6/Growth Hormone; 9034-39-3/Growth Hormone-Releasing Hormone

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