Document Detail


Phorbol ester-mediated downregulation of tropoelastin expression is controlled by a posttranscriptional mechanism.
MedLine Citation:
PMID:  1637804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of tropoelastin, the principal precursor of elastic fibers, is tissue-specific and is limited to a brief developmental period. Little is known, however, about the mechanisms that regulate the tissue- and temporal-specific expression of elastogenesis. The tropoelastin promoter contains putative phorbol ester responsive elements, or AP-1 binding sites, but the functional significance of these sequences is unknown. To test if tropoelastin expression is influenced by phorbol esters, we exposed elastogenic fetal bovine chondrocytes to 10(-7) M 12-O-tetradecanoylphorbol 13-acetate (TPA). Tropoelastin mRNA levels decreased greater than 10-fold in response to TPA, and this downregulation was paralleled by a decline in the secretion of tropoelastin protein into the culture medium. As determined by nuclear-runoff assay and transient transfection with a human gene promoter-CAT construct, tropoelastin transcription was unaffected after exposure to TPA. As indicated by actinomycin D experiments, the half-life of tropoelastin mRNA in control cells was about 20 h, but exposure to TPA resulted in an accelerated decay of the tropoelastin transcript (t1/2 = 2.2 h). These data indicate that downregulation of tropoelastin expression was controlled by a posttranscriptional mechanism and that the AP-1 elements in the bovine tropoelastin promoter may not be involved in regulation of production.
Authors:
W C Parks; M E Kolodziej; R A Pierce
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  31     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6639-45     Citation Subset:  IM    
Affiliation:
Division of Dermatology, Jewish Hospital, Washington University Medical Center, St. Louis, Missouri 63110.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage / drug effects,  physiology*
Cattle
Cells, Cultured
Chloramphenicol O-Acetyltransferase / genetics,  metabolism
Dactinomycin / pharmacology
Fetus
Gene Expression Regulation / drug effects*
Humans
Kinetics
Promoter Regions, Genetic
Proto-Oncogene Proteins c-jun / metabolism
RNA Processing, Post-Transcriptional* / drug effects
RNA, Messenger / genetics,  metabolism*
Tetradecanoylphorbol Acetate / pharmacology*
Time Factors
Transcription, Genetic / genetics
Transfection
Tropoelastin / genetics*
Grant Support
ID/Acronym/Agency:
AR-07824/AR/NIAMS NIH HHS; HL-41040/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Tropoelastin; 16561-29-8/Tetradecanoylphorbol Acetate; 50-76-0/Dactinomycin; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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