| Phorbol ester-induced monocytic differentiation is associated with G2 delay and down-regulation of cdc25 expression. | |
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MedLine Citation:
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PMID: 8466854 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Treatment of human U-937 myeloid leukemia cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) is associated with growth arrest and induction of monocytic differentiation. The present results demonstrate that exposure of G1- or S phase-enriched U-937 cells to TPA was associated with delayed transition through G2-M and then exit from the cell cycle in the subsequent G0-G1 phase. In contrast, G2-M cells treated with TPA progressed through G1-S, were delayed in the next G2-M phase, and then entered a nonproliferative G0-G1 state. The effects of TPA on expression of cell cycle control genes demonstrated down-regulation of the cdc25 mitotic inducer during S-G2 progression. In order to investigate the role of cdc25 in the control of cell cycle progression, U-937 cells were stably transfected with an antisense cdc25 construct under control of a metallothionein-inducible promoter. Expression of this construct in G1 phase cells was associated with a significant delay in transition through S and G2-M phase. Similar results were obtained when G1 phase cells were incubated with a 14-base pair antisense cdc25 oligomer. Taken together, these results indicate that the cdc25 gene product is functionally associated with S-G2 transition of proliferating myeloid leukemia cells and that down-regulation of this gene by TPA is associated with G2-M delay. |
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Authors:
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R Hass; H Gunji; M Hirano; R Weichselbaum; D Kufe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research Volume: 4 ISSN: 1044-9523 ISO Abbreviation: Cell Growth Differ. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-05-11 Completed Date: 1993-05-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9100024 Medline TA: Cell Growth Differ Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 159-66 Citation Subset: IM |
Affiliation:
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Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Differentiation / drug effects Down-Regulation / drug effects G2 Phase / drug effects* Gene Expression / drug effects* Humans Leukemia, Myeloid / drug therapy*, genetics Molecular Sequence Data Monocytes / cytology, drug effects* S Phase / drug effects* Tetradecanoylphorbol Acetate / pharmacology* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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CA42802/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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