Document Detail

Phorbol ester-induced effects on cell cycle progression and terminal deoxynucleotidyltransferase (TdT) activity in KM-3 pre-B cell line.
MedLine Citation:
PMID:  8514336     Owner:  NLM     Status:  MEDLINE    
Phorbol myristic acetate (PMA) is a tumor-promoting agent that has been shown to induce differentiation of human leukemia cells and of normal lymphoid cells. We have investigated the ability of PMA to induce inhibition of cell growth of the human KM-3 pre-B leukemic cell line by multiparametric analysis. Our results show that PMA treatment induces cell differentiation with the disappearance of terminal deoxynucleotidyltransferase and a decrease of cell growth, as evaluated by [3H]thymidine uptake. Flow cytometric analysis of BrdU incorporation shows that PMA is able to induce a modification of the cell cycle with a sharp decrease of the percentage of S-phase cells, which is more evident after 24 h of treatment. Comparison between the cell growth kinetics and TdT synthesis and activity shows that differentiated cells are still able to proliferate to a certain extent and that the TdT disappearance and the initial decrease of cell proliferation are two independent effects of PMA.
O Trubiani; R Di Primio; L Zamai; D Bosco; F J Bollum; M Vitale
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  35     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-07-22     Completed Date:  1993-07-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  265-9     Citation Subset:  IM    
Istituto di Citomorfologia del C.N.R., Facoltà di Medicina, Università di Chieti.
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MeSH Terms
B-Lymphocytes / drug effects,  enzymology*
Cell Cycle / drug effects
Cell Differentiation
Cell Division
DNA Nucleotidylexotransferase / metabolism*
DNA Replication
Flow Cytometry
Immunoenzyme Techniques
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / enzymology
Tetradecanoylphorbol Acetate / pharmacology*
Thymidine / metabolism
Tumor Cells, Cultured
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 50-89-5/Thymidine; EC Nucleotidylexotransferase

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