| Phorbol ester- and calcium-induced reorganization of 180-kDa bullous pemphigoid antigen on the ventral surface of cultured human keratinocytes as studied by immunofluorescence and immunoelectron microscopy. | |
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MedLine Citation:
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PMID: 1426040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hemidesmosome is an adhesion structure of the epidermal-dermal junction in keratinocytes. When keratinocytes migrate laterally or upward to differentiate, they must control the formation and disintegration of the hemidesmosomes. When keratinocytes are cultured in low-calcium (below 0.1 mM) medium, all cells behave like basal cells, adhere to the culture dish, and proliferate without differentiation. The calcium addition induces the differentiation. A bullous pemphigoid antigen, 180-kDa BPA, has been shown to be a component of the hemidesmosome. Using a monoclonal antibody to the 180-kDa BPA and a human squamous cell carcinoma cell line (DJM-1 cells), the fate of hemidesmosomes was studied after the addition of calcium to low-calcium-grown cells and 12-tetradecanoylphorbol-13-acetate (TPA) to high-calcium (1.87 mM) grown cells by immunofluorescence and immunoelectron microscopy. The antigen was distributed evenly as fine dots on the entire ventral surface of low-calcium cells, whereas they formed a peculiar, concentric ring or arch arrangement on the ventral surface of high-calcium cells. Immunoelectron microscopy revealed the deposits of gold particles at sites on the membrane surface, where some filamentous or electron-dense materials were associated, although the complete structure of hemidesmosomes was not formed. They deposited directly onto the membrane surface in low-calcium cells and with a distance of 20-50 nm from the membrane surface in high-calcium cells. The calcium addition caused a profound reduction of the 180-kDa BPA-positive area for 30 to 120 min and then formed the high-calcium-ring pattern after 4 to 6 h. A similar calcium response was seen in normal human keratinocytes. TPA (16 nM) treatment caused disintegration of the ring pattern in high-calcium DJM-1 cells. This was inhibited with a protein kinase C (PKC) inhibitor. H7 (20 microM). These results suggest that the hemidesmosome is a dynamic structure and PKC can be one of the major factors in controlling the hemidesmosome, since it is known that the low-high calcium shift induces a calcium influx and a PKC activation, and TPA activates PKC in keratinocytes. |
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Authors:
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Y Kitajima; K Owaribe; Y Nishizawa; Y Jokura; H Yaoita |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental cell research Volume: 203 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-12-04 Completed Date: 1992-12-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 17-24 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Jichi Medical School, Tochigiken, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Autoantigens / analysis, metabolism* Calcium / pharmacology* Carcinoma, Squamous Cell Carrier Proteins* Cell Membrane / drug effects, metabolism, ultrastructure Cells, Cultured Collagen* Cytoskeletal Proteins* Desmosomes / drug effects, metabolism*, ultrastructure Fluorescent Antibody Technique Humans Isoquinolines / pharmacology Keratinocytes / drug effects, metabolism*, pathology Microscopy, Immunoelectron Molecular Weight Nerve Tissue Proteins* Non-Fibrillar Collagens* Pemphigoid, Bullous / pathology* Piperazines / pharmacology Protein Kinase C / antagonists & inhibitors Skin Neoplasms Tetradecanoylphorbol Acetate / pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/DST protein, human; 0/Isoquinolines; 0/Nerve Tissue Proteins; 0/Non-Fibrillar Collagens; 0/Piperazines; 0/collagen type XVII; 16561-29-8/Tetradecanoylphorbol Acetate; 7440-70-2/Calcium; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; 9007-34-5/Collagen; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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