Document Detail


Phloroglucinol protects human keratinocytes from ultraviolet B radiation by attenuating oxidative stress.
MedLine Citation:
PMID:  23126295     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Ultraviolet B generates reactive oxygen species by interacting with water in irradiated skin tissues and cells in turn causes lipid peroxidation, protein modification and further DNA damage.
PURPOSE: This study examined the cytoprotective effects of phloroglucinol (1,3,5-trihydroxybenzene) on ultraviolet B-irradiated cultured human keratinocytes.
METHODS: The human keratinocyte cell line HaCaT cells were treated with 10 μM of phloroglucinol. After 1 h, the cells were irradiated with ultraviolet B light at 30 mJ/cm(2) and incubated at 37°C.
RESULTS: Phloroglucinol scavenged both the superoxide anion and hydroxyl radical in a cell-free system and ultraviolet B-induced intracellular reactive oxygen species. Phloroglucinol reduced ultraviolet B-generated lipid peroxidation, protein modification and DNA strand breaks. The enzymatic effects of phloroglucinol restored cellular glutathione levels, superoxide dismutase and catalase activities, which were impaired by ultraviolet B radiation.
CONCLUSION: Phloroglucinol provides the protective effects in human keratinocyte cell line exposed to ultraviolet B radiation, suggesting that phloroglucinol can be used as a photoprotective agent.
Authors:
Ki Cheon Kim; Mei Jing Piao; Suk Ju Cho; Nam Ho Lee; Jin Won Hyun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photodermatology, photoimmunology & photomedicine     Volume:  28     ISSN:  1600-0781     ISO Abbreviation:  Photodermatol Photoimmunol Photomed     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013641     Medline TA:  Photodermatol Photoimmunol Photomed     Country:  England    
Other Details:
Languages:  eng     Pagination:  322-31     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju, Korea.
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