Document Detail

Philadelphia chromosome-positive blastic leukaemia: ultrastructural and ultracytochemical evidence of basophil and mast cell differentiation.
MedLine Citation:
PMID:  6291577     Owner:  NLM     Status:  MEDLINE    
Ultrastructural, ultracytochemical, immunologic and biochemical studies were performed on leukaemic cells from 41 patients with Philadelphia chromosome-positive blastic leukaemia; 28 patients were in blast transformation of chronic myelogenous leukaemia and 13 patients presented with 'acute' leukaemia. The patients were divided into two morphologic groups, lymphoid (16 cases) and myeloid (25 cases), on the basis of light microscopy and cytochemistry. All lymphoid cases studied for the presence of CALLA (10 patients) and TdT (11 patients) were positive. Two of 13 myeloid cases studied were TdT positive. The blasts from 10 of 16 lymphoid cases contained immature basophil/mast cell granules on ultrastructural examination. Peroxidase-positive 'lymphoid' blasts were noted in three of seven patients studied by ultracytochemical techniques. The reactivity was primarily confined to granular structures. Of the 25 cases in the myeloid group, blasts from 14 cases showed basophil/mast cell differentiation, nine cases showed neutrophil/monocyte features, and two cases were megakaryoblastic. Distinct patterns of ultrastructural peroxidase positivity were seen in the seven myeloid cases studied. In basophil/mast cell precursors the reactivity was primarily confined to granules; neutrophil precursors showed reactivity in the nuclear envelope, rough endoplasmic reticulum (RER), golgi and granules; in megakaryoblasts, only the nuclear envelope and RER were positive while the granules were consistently negative.
J L Parkin; R W McKenna; R D Brunning
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  52     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-01-27     Completed Date:  1983-01-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  663-77     Citation Subset:  IM    
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MeSH Terms
Basophils / enzymology,  ultrastructure*
Blood Platelets / enzymology
Bone Marrow / enzymology,  ultrastructure
Cell Differentiation
Chromosomes, Human, 21-22 and Y / ultrastructure*
Cytoplasmic Granules / ultrastructure
Leukemia, Lymphoid / enzymology,  ultrastructure*
Mast Cells / enzymology,  ultrastructure*
Microscopy, Electron
Peroxidase / analysis
Peroxidases / analysis
Reg. No./Substance:
EC 1.11.1.-/Peroxidases; EC

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