Document Detail


Phenyl propenoic side chain degradation of ferulic acid by Pycnoporus cinnabarinus - elucidation of metabolic pathways using [5-2H]-ferulic acid.
MedLine Citation:
PMID:  11173097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The white-rot fungus Pycnoporous cinnabarinus (DMS-1184) was submerged cultured for 22 days under controlled conditions in a bioreactor. After 6, 9, and 15 days of culture the growth medium was supplemented with [5-2H]-labelled ferulic acid (I). The major phenolic compounds identified labelled were four lignans, the methyl esters of ferulic (I) and vanillic acid (VIII), (E)-coniferyl aldehyde (II), (E)-coniferyl alcohol (III), vanillic acid (VIII), vanillin (IX) and vanillyl alcohol (X). The detection of considerable amounts of labelled 4-hydroxy-3-methoxyacetophenone (VII) in the late growth phase suggested the increasing formation and decarboxylation of free 4-hydroxy-3-methoxybenzoylacetic acid (VI) and, thus, a beta-oxidation-like degradation of ferulic acid (I) or its methyl ester to vanillic acid (VIII). 4-Hydroxy-3-methoxybenzoylacetic acid methyl ester (VI) and 3-hydroxy-(4-hydroxy-3-methoxyphenyl)-propanoic acid methyl ester (V) were synthesised and then identified as metabolites in the culture medium. The fungal degradation of the phenyl propenoic side chain of ferulic acid (I), a principal key step of lignin decomposition, appeared to proceed analogous to fatty acids.
Authors:
U Krings; S Pilawa; C Theobald; R G Berger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biotechnology     Volume:  85     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  305-14     Citation Subset:  IM    
Affiliation:
Institut für Lebensmittelchemie, Zentrum Angewandte Chemie der Universität, Wunstorfer Str. 14, D 30453 Hannover, Germany
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MeSH Terms
Descriptor/Qualifier:
Basidiomycota / metabolism*
Benzaldehydes / metabolism
Bioreactors*
Biotechnology
Biotransformation
Chromatography, High Pressure Liquid
Coumaric Acids / chemistry,  metabolism*
Gas Chromatography-Mass Spectrometry
Chemical
Reg. No./Substance:
0/Benzaldehydes; 0/Coumaric Acids; 1135-24-6/ferulic acid; 121-33-5/vanillin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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