Document Detail


Phenotypic variation of Pseudomonas brassicacearum as a plant root-colonization strategy.
MedLine Citation:
PMID:  15305608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas brassicacearum was isolated as a major root-colonizing population from Arabidopsis thaliana. The strain NFM421 of P. brassicacearum undergoes phenotypic variation during A. thaliana and Brassica napus root colonization in vitro as well as in soil, resulting in different colony appearance on agar surfaces. Bacteria forming translucent colonies (phase II cells) essentially were localized at the surface of young roots and root tips, whereas wild-type cells (phase I cells) were localized at the basal part of roots. The ability of phase II cells to spread and colonize new sites on root surface correlates with over-production of flagellin as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of surface proteins and microsequencing. Moreover, phase II cells showed a higher ability to swim and to swarm on semisolid agar medium. Phase I and phase II cells of P. brassicacearum NFM421 were tagged genetically with green fluorescent protein and red fluorescent protein. Confocal scanning laser microscopy was used to localize phase II cells on secondary roots and root tips of A. thaliana, whereas phase I cells essentially were localized at the basal part of roots. These experiments were conducted in vitro and in soil. Phenotypic variation on plant roots is likely to be a colonization strategy that may explain the high colonization power of P. brassicacearum.
Authors:
Wafa Achouak; Sandrine Conrod; Valérie Cohen; Thierry Heulin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  17     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-12     Completed Date:  2004-11-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  872-9     Citation Subset:  IM    
Affiliation:
Laboratoire d'Ecologie Microbienne de la Rhizosphere (LEMiR), UMR 6191 CNRS-CEA-Université Aix-Marseille II, DEVM-DSV, IFR 112 PMSE, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France. wafa.achouak@cea.fr
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  microbiology*
Colony Count, Microbial
Culture Media
Flagellin / metabolism
Genetic Variation
Phenotype
Plant Roots / genetics,  microbiology*
Plasmids
Pseudomonas / classification,  genetics*,  pathogenicity
Soil Microbiology*
Chemical
Reg. No./Substance:
0/Culture Media; 12777-81-0/Flagellin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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