Document Detail


Phenotypic switching to long cilia effected by various proteases: results with Dendraster excentricus and Stronglyocentrotus purpuratus blastulae.
MedLine Citation:
PMID:  3641879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Incubation in trypsin effects a phenotypic switch from short to long cilia (greater than 30 micron) in hatched blastulae of the sea urchin, Arbacia punctulata. To determine how trypsin causes such a switch we tested whether the phenomenon was unique to the species, Arbacia, and to the protease, trypsin. With two other echinoderm species, the sand dollar, Dendraster excentricus, and the sea urchin, Strongylocentrotus purpuratus, trypsin incubation increased the percentage of long cilia. During incubation of D. excentricus in trypsin, the percentage of long cilia increased progressively from the normal 10% long cilia of the apical tuft to 45-50% long cilia covering 1/2-3/4 of the embryo. With S. purpuratus blastulae, however, the percentage of long cilia was lower (32-40%) and the results were more variable. Of the additional proteases tested with D. excentricus, elastase was more effective than trypsin in terms of the percentage of long cilia (58%), the mean length, and the broad distribution of lengths formed. Thermolysin was about as effective as trypsin but chymotrypsin was much less so. Thus, increases in ciliary length were not unique to a particular echinoderm species or to incubation in trypsin. The magnitude of the change in length distribution, however, was species- and enzyme-dependent. An extracellular or membrane component with differential susceptibility to various proteases may, therefore, be involved in altering ciliary length.
Authors:
M A Riederer-Henderson
Related Documents :
22863099 - Performance and metabolic responses of holstein calves to supplemental chromium in colo...
21902859 - The proportion of lycopene isomers in human plasma is modulated by lycopene isomer prof...
21602259 - The changes in plasma retinol-binding protein 4 levels are associated with those of the...
22891219 - Fto genotype and 2-year change in body composition and fat distribution in response to ...
22696509 - Maternal high-fat diet programs wnt genes through histone modification in the liver of ...
22436279 - Effects of alfalfa silage storage structure and roasting corn on ruminal digestion and ...
19951259 - Countervailing vascular effects of rosiglitazone in high cardiovascular risk mice: role...
3006519 - Dissociation of sympathetic and thermogenic activity in brown fat of syrian hamsters.
25336449 - In utero exposure to prepregnancy maternal obesity and postweaning high-fat diet impair...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  240     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-02-12     Completed Date:  1987-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  327-33     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / ultrastructure
Chymotrypsin / metabolism
Cilia / ultrastructure*
Membrane Proteins / physiology
Microscopy, Electron, Scanning
Pancreatic Elastase / metabolism
Sea Urchins / embryology*
Species Specificity
Thermolysin / metabolism
Time Factors
Trypsin / metabolism
Chemical
Reg. No./Substance:
0/Membrane Proteins; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.36/Pancreatic Elastase; EC 3.4.21.4/Trypsin; EC 3.4.24.27/Thermolysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chimaerism of primordial germ cells in the early postimplantation mouse embryo following microsurgic...
Next Document:  Early detection of preterm labor: ambulatory home monitoring of uterine activity.