Document Detail


Phenotypic stress response of Pseudomonas aeruginosa following culture in water microcosms.
MedLine Citation:
PMID:  22361708     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The purpose of the present study was to explore the potential behavioural changes of Pseudomonas aeruginosa following growth in different aquatic environmental conditions. To achieve this, P. aeruginosa was cultured in various water microcosms for 12 months under fixed (pH, nutrients and temperature) factors. P. aeruginosa responses to these conditions were investigated using colony morphotype, biochemical and enzymatic characterisation, pyocin typing, serotyping, sensitivity to different classes of antibiotics and molecular identification. Results show that starvation in water microcosms lead to unusual phenotypes. Of interest is that the pyocin changed from 24/n in the wild type to 83/a following culture in the water microcosms, and the serotype changed from O6 in the wild type to O1 in microcosm-cultured P. aeruginosa. Furthermore, the starvation period in various aquatic microcosms enhanced the resistance of P. aeruginosa against beta-lactam antibiotics. Compared to the other aquatic environments, the seawater microcosm produced the greatest amount of variations in P. aeruginosa. Overall, data demonstrated a high adaptability of P. aeruginosa to environmental changes. This may explain the unusual antibiotic-resistant phenotypes belonging to P. aeruginosa species, and their capacity for spreading that leads to human infections.
Authors:
Jihane Cheriaa; Mahmoud Rouabhia; Makaoui Maatallah; Amina Bakhrouf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of water and health     Volume:  10     ISSN:  1477-8920     ISO Abbreviation:  J Water Health     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101185420     Medline TA:  J Water Health     Country:  England    
Other Details:
Languages:  eng     Pagination:  130-9     Citation Subset:  IM    
Affiliation:
Laboratory of Analysis, Treatment, Valorisation and Environmental Pollution and Products 'LR01ES16', Faculty of Pharmacy, Monastir Avicenne Street, 5000 Monastir, Tunisia E-mail: jihane_cheriaa@yahoo.fr.
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