Document Detail

Phenotypic and metabolic characteristics of maternal monocytes and granulocytes in preterm labor with intact membranes.
MedLine Citation:
PMID:  11717645     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Experimental and clinical studies support a role for the fetus in the control of the onset of labor. Fetal systemic inflammation, but not a maternal inflammatory response, has been linked to the onset of preterm labor and delivery on the basis of the determination of inflammatory cytokines in fetal and maternal blood. We propose that parturition requires fetomaternal cooperation and that inflammation is an integral part of the parturitional process. This study used flow cytometry, a sensitive technique for the detection of intravascular inflammation, to assess whether maternal inflammation is present in preterm labor. STUDY DESIGN: A prospective cross-sectional study was performed including patients with preterm labor (n = 55) and women with normal pregnancy (n = 50). Intravascular inflammation was studied by using flow cytometry. Maternal blood was assayed to determine granulocyte and monocyte phenotype by using monoclonal antibodies, which included the following cluster of differentiation (CD) markers: CD11b, CD14, CD15, CD16, CD18, CD49d, CD62L, CD64, CD66b, and HLA-DR. Oxidative burst and generation of basal intracellular oxygen radical species were assessed. Statistical analysis was conducted with the use of nonparametric methods. A P value of <.01 was considered statistically significant. RESULTS: Preterm labor was associated with a significant increase in the median mean channel brightness of CD11b, CD15, and CD66b on granulocytes and median mean channel brightness of CD11b and CD15 on monocytes. The ratio of oxidative burst over basal intracellular oxygen radical species in both granulocytes and monocytes was increased in preterm labor (P <. 01). CONCLUSION: Preterm labor with intact membranes is associated with phenotypic and metabolic changes of maternal granulocytes and monocytes.
M T Gervasi; T Chaiworapongsa; N Naccasha; S Blackwell; B H Yoon; E Maymon; R Romero
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  185     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-21     Completed Date:  2001-12-19     Revised Date:  2005-11-21    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1124-9     Citation Subset:  AIM; IM    
Perinatology Research Branch, National Institute of Child Health and Human Development, Bethesda, Md, USA.
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MeSH Terms
Antigens, CD
Antigens, CD15 / analysis
Antigens, Neoplasm*
Cell Adhesion Molecules*
Cross-Sectional Studies
Extraembryonic Membranes / physiopathology*
Granulocytes / physiology*
Macrophage-1 Antigen / analysis
Membrane Glycoproteins / analysis
Monocytes / physiology*
Obstetric Labor, Premature / blood*,  physiopathology*
Pregnancy / blood*
Prospective Studies
Reactive Oxygen Species / blood
Reference Values
Respiratory Burst
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD15; 0/Antigens, Neoplasm; 0/CEACAM8 protein, human; 0/Cell Adhesion Molecules; 0/Macrophage-1 Antigen; 0/Membrane Glycoproteins; 0/Reactive Oxygen Species

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