Document Detail


Phenotypic identification of Actinomyces and related species isolated from human sources.
MedLine Citation:
PMID:  11682514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent advancements in chemotaxonomic and molecular biology-based identification methods have clarified the taxonomy of the genus Actinomyces and have led to the recognition of several new Actinomyces and related species. Actinomyces-like gram-positive rods have increasingly been isolated from various clinical specimens. Thus, an easily accessible scheme for reliable differentiation at the species level is needed in clinical and oral microbiology laboratories, where bacterial identification is mainly based on conventional biochemical methods. In the present study we designed a two-step protocol that consists of a flowchart that describes rapid, cost-efficient tests for preliminary identification of Actinomyces and closely related species and an updated more comprehensive scheme that also uses fermentation reactions for accurate differentiation of Actinomyces and closely related species.
Authors:
N Sarkonen; E Könönen; P Summanen; M Könönen; H Jousimies-Somer
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  39     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-10-29     Completed Date:  2002-03-13     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3955-61     Citation Subset:  IM    
Affiliation:
Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland. nanna.sarkonen@ktl.fi
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MeSH Terms
Descriptor/Qualifier:
Actinomyces / classification*,  isolation & purification,  metabolism*
Actinomycetales Infections / microbiology*
Bacterial Typing Techniques / economics,  methods*
Enzymes / metabolism
Fermentation
Humans
Hydrolysis
Phenotype
Reagent Kits, Diagnostic
Chemical
Reg. No./Substance:
0/Enzymes; 0/Reagent Kits, Diagnostic
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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