| Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241). | |
| | |
MedLine Citation:
|
PMID: 14523526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phytic acid, myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the major storage compound of phosphorous (P) in plants, predominantly accumulating in seeds (up to 4-5% of dry weight) and pollen. In cereals, phytic acid is deposited in embryo and aleurone grain tissues as a mixed "phytate" salt of potassium and magnesium, although phytates contain other mineral cations such as iron and zinc. During germination, phytates are broken down by the action of phytases, releasing their P, minerals and myo-inositol which become available to the growing seedling. Phytic acid represents an anti-nutritional factor for animals, and isolation of maize low phytic acid ( lpa) mutants provides a novel approach to study its biochemical pathway and to tackle the nutritional problems associated with it. Following chemical mutagenesis of pollen, we have isolated a viable recessive mutant named lpa 241 showing about 90% reduction of phytic acid and about a tenfold increase in seed-free phosphate content. Although germination rate was decreased by about 30% compared to wild-type, developement of mutant plants was apparentely unaffected. The results of the genetic, biochemical and molecular characterization experiments carried out by SSR mapping, MDD-HPLC and RT-PCR are consistent with a mutation affecting the MIPS1S gene, coding for the first enzyme of the phytic acid biosynthetic pathway. |
| | |
Authors:
|
R Pilu; D Panzeri; G Gavazzi; S K Rasmussen; G Consonni; E Nielsen |
Related Documents
:
|
17090936 - Caenorhabditis elegans mutants having altered preference of chemotaxis behavior during ... 2227406 - Branched chain amino acid regulation of the ilv2 locus in saccharomyces cerevisiae. 22186046 - Effect of vanillin, ethyl vanillin, and vanillic acid on the growth and heat resistance... 3038076 - Pseudomonas mutant strains that accumulate androstane and seco-androstane intermediates... 22882126 - The effect of an amphiphilic self-assembled lipid lamellar phase on the relief of dry s... 15738536 - Zoledronic acid significantly reduces skeletal complications compared with placebo in j... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-10-02 |
Journal Detail:
|
Title: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik Volume: 107 ISSN: 0040-5752 ISO Abbreviation: Theor. Appl. Genet. Publication Date: 2003 Oct |
Date Detail:
|
Created Date: 2003-10-07 Completed Date: 2004-03-12 Revised Date: 2008-02-15 |
Medline Journal Info:
|
Nlm Unique ID: 0145600 Medline TA: Theor Appl Genet Country: Germany |
Other Details:
|
Languages: eng Pagination: 980-7 Citation Subset: IM |
Affiliation:
|
Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Crops, Agricultural Genotype Humans Molecular Structure Mutation Pedigree Phenotype Phosphates / metabolism Phytic Acid / chemistry, metabolism* Seeds / physiology* Zea mays / cytology, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Phosphates; 83-86-3/Phytic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Complex population genetic structure in the endemic Canary Island pine revealed using chloroplast mi...
Next Document: Grass consensus STS markers: an efficient approach for detecting polymorphism in Lolium.