Document Detail


Phenotypic and functional characterization of human CD25+ B cells.
MedLine Citation:
PMID:  16556269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We demonstrate that humans have a phenotypically and functionally distinct subset of B lymphocytes that express the interleukin (IL)-2 receptor (IL-2R) alpha-chain, cluster of differentiation (CD) 25. We found that one-third of the circulating CD20+ B cells expressed CD25 and, using fluorescence-activated cell sorter (FACS) analysis, that these cells were significantly larger and more granulated than B cells not expressing CD25. The simultaneous expression of the other two subunits (CD122 and CD132) and the proliferative responses of cells expressing CD25 to IL-2 suggested that, in addition to CD25, functional IL-2 receptors were expressed on this cell population. CD25 expression on B cells was selectively up-regulated by Toll-like receptor 2 (TLR2), TLR4, and TLR9 ligands but not by a TLR3 ligand or Epstein-Barr virus (EBV) stimulation. Blockade of the nuclear factor (NF)-kappaB pathway completely abolished CD25 up-regulation by these B cells. Interestingly, CD25+ B cells expressed significantly higher levels of surface immunoglobulins but lacked the ability to secrete immunoglobulin (Ig), as compared with CD25- B cells. Furthermore, CD25+ B cells performed significantly better as antigen-presenting cells in allogeneic mixed lymphocyte reactions (MLR), which may be a result of their expression of high levels of the costimulatory molecules CD27 and CD80. Finally, blocking of CD25 on B cells led to an almost total abrogation of MLR. Our results indicate that CD25+ B cells have distinct phenotypic and functional properties, including the ability to contribute to antigen presentation, which is linked to their expression of CD25. Finally, the differential regulation of CD25 expression via selective TLR ligands suggests a role for CD25+ B cells in bridging innate and acquired immune responses.
Authors:
Mikael Brisslert; Maria Bokarewa; Pia Larsson; Kajsa Wing; L Vincent Collins; Andrej Tarkowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  117     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-24     Completed Date:  2006-05-03     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  548-57     Citation Subset:  IM    
Affiliation:
Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at Gôteborg University, Gôteborg, Sweden. mikael.brisslert@rheuma.gu.se
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigen Presentation / immunology
B-Lymphocyte Subsets / immunology*
Cell Separation / methods
Flow Cytometry / methods
Herpesvirus 4, Human / immunology
Humans
Immunoglobulins / biosynthesis
Immunophenotyping / methods
Interleukin-2 / immunology
Ligands
Lymphocyte Culture Test, Mixed
Mitogens / immunology
Receptors, Interleukin-2 / blood*
Toll-Like Receptors / immunology
Chemical
Reg. No./Substance:
0/Immunoglobulins; 0/Interleukin-2; 0/Ligands; 0/Mitogens; 0/Receptors, Interleukin-2; 0/Toll-Like Receptors
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