Document Detail


Phenotypic and functional characteristics of human newborns' B lymphocytes.
MedLine Citation:
PMID:  1688576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been demonstrated two major facts concerning human newborns' B lymphocytes: 1) they differentiate poorly into Ig-producing cells and 2) they express CD5 and CD1c membrane proteins. We have further analyzed human newborns' B cell characteristics and found that approximately half of them express activation Ag, i.e., 4F2 and IL-2R, both associated in significant proportions with CD23 and Bac-1. These membrane Ag were found both on CD5(+) and CD5(-) B cells. Newborns' B cells do not exhibit other activation markers because they express surface IgD, and because their size, RNA, and DNA contents do not differ from those of adults' B cells, indicating that they are in the G0/G1 cell cycle phase. Newborns' B cell proliferation can be induced by rIL-2, rIL-4, low m.w. B cell growth factor, and by Staphylococcus aureus protein A. It is presently difficult to build a hypothesis accounting for all the specific findings made on newborns' B cells. It is not known for instance whether CD5(+) and (-) B cells belong to distinct subsets as suggested by the fluorescence intensity curve obtained with an anti-CD5 antibody or to distinct stages in a unique pattern of B cell maturation during fetal and newborn life. This may indicate that partially activated B cells actually produce natural polyspecific autoantibodies of the IgM isotype found in newborns' human serum.
Authors:
A Durandy; L Thuillier; M Forveille; A Fischer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  144     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-09     Completed Date:  1990-02-09     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  60-5     Citation Subset:  AIM; IM    
Affiliation:
INSERM U 132, Hôpital des Enfants Malades, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Antigens, CD5
Antigens, Differentiation / analysis
B-Lymphocytes / cytology,  immunology,  physiology*
Cell Differentiation
Cell Membrane / immunology
Flow Cytometry
Humans
Immunoglobulin D / analysis
Infant, Newborn / immunology*
Interleukin-2 / pharmacology
Interleukin-4 / pharmacology
Lymphocyte Activation / drug effects
Receptors, Antigen, B-Cell / analysis
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD5; 0/Antigens, Differentiation; 0/Immunoglobulin D; 0/Interleukin-2; 0/Receptors, Antigen, B-Cell; 207137-56-2/Interleukin-4

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