Document Detail


Phenotypic characterization of taste cells of the mouse small intestine.
MedLine Citation:
PMID:  17290008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nutrient-evoked gastrointestinal reflexes are likely initiated by specialized epithelial cells located in the small intestine that detect luminal stimuli and release mediators that activate vagal endings. The G-protein alpha-gustducin, a key signal molecule in lingual taste detection, has been identified in mouse small intestine, where it may also subserve nutrient detection; however, the phenotype of alpha-gustducin cells is unknown. Immunohistochemistry was performed throughout the mouse small intestine for alpha-gustducin, enteroendocrine cell markers 5-HT and glucagon-like peptide-1 (GLP-1), and brush cell markers neuronal nitric oxide synthase and Ulex europaeus agglutinin-1 (UEA-1) lectin binding, singly, and in combination. alpha-Gustducin was expressed in solitary epithelial cells of the mid to upper villus, which were distributed in a regional manner with most occurring within the midjejunum. Here, 27% of alpha-gustducin cells colabeled for 5-HT and 15% for GLP-1; 57% of alpha-gustducin cells colabeled UEA-1, with no triple labeling. alpha-Gustducin cells that colabeled for 5-HT or GLP-1 were of distinct morphology and exhibited a different alpha-gustducin immunolabeling pattern to those colabeled with UEA-1. Neuronal nitric oxide synthase was absent from intestinal epithelium despite strong labeling in the myenteric plexus. We conclude that subsets of enteroendocrine cells in the midjejunum and brush cells (more generally distributed) are equipped to utilize alpha-gustducin signaling in mice. Intestinal taste modalities may be signaled by these enteroendocrine cells via the release of 5-HT, GLP-1, or coexpressed mediators or by brush cells via a nonnitrergic mediator in distinct regions of the intestine.
Authors:
Kate Sutherland; Richard L Young; Nicole J Cooper; Michael Horowitz; L Ashley Blackshaw
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-08
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  292     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-10     Completed Date:  2007-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G1420-8     Citation Subset:  IM    
Affiliation:
Discipline of Physiology, School of Molecular and Biomedical Sciences, University of Adelaide, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Enteroendocrine Cells / metabolism*
Gene Expression
Glucagon-Like Peptide 1 / metabolism
Immunohistochemistry
Intestinal Mucosa / metabolism
Intestine, Small / chemistry*,  cytology
Male
Mice
Mice, Inbred C57BL
Nitric Oxide Synthase Type I / metabolism
Phenotype
Plant Lectins / metabolism
Serotonin / metabolism
Transducin / physiology*
Chemical
Reg. No./Substance:
0/Plant Lectins; 0/Ulex europaeus lectins; 147979-21-3/Gnat3 protein, rat; 50-67-9/Serotonin; 89750-14-1/Glucagon-Like Peptide 1; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nos1 protein, mouse; EC 3.6.1.-/Transducin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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