| Phenotypic characterization of Corynebacterium glutamicum using elementary modes towards synthesis of amino acids. | |
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MedLine Citation:
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PMID: 22132055 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Elementary flux mode (EFM) analysis is a powerful tool to represent the metabolic network structure and can be further utilized for flux analysis. The method enables characterization and quantification of feasible phenotypes in microbes. EFM analysis was employed to characterize the phenotype of Corynebacterium glutamicum to yield various amino acids. The metabolic network of C. glutamicum yielded 62 elementary modes by incorporating the accumulation of amino acids namely, lysine, alanine, valine, glutamine and glutamate. The analysis also allowed us to compute the maximum theoretical yield for the synthesis of various amino acids. These 62 elementary modes were further used to obtain optimal phenotypic space towards accumulation of biomass and lysine. The study indicated that the optimal solution space from 62 elementary modes forms a super space which incorporates various mutants including lysine producing strain of C. glutamicum. The analysis was also extended to obtain sensitivity of the network to variation in the stoichiometry of NADP in the definition of biomass. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11693-011-9073-8) contains supplementary material, which is available to authorized users. |
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Authors:
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Devesh Radhakrishnan; Meghna Rajvanshi; K V Venkatesh |
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Publication Detail:
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Type: Journal Article Date: 2011-02-22 |
Journal Detail:
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Title: Systems and synthetic biology Volume: 4 ISSN: 1872-5333 ISO Abbreviation: Syst Synth Biol Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-12-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101300404 Medline TA: Syst Synth Biol Country: Germany |
Other Details:
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Languages: eng Pagination: 281-91 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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