Document Detail

Phenotypic characteristics of hybrid cells produced by cell fusion of porcine adrenal chromaffin cells with human mesenchymal stem cells: a preliminary study.
MedLine Citation:
PMID:  18252039     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: Transplantation of adrenal chromaffin cells (CCs) that release endogenous opioid peptides and catecholamines produces significant antinociceptive effects in patients with terminal cancer pain. In clinical practice, however, obtaining a sufficient number of chromaffin cells may not be possible because of the limited availability of human adrenal tissue. Recent works have shown that fusion of bone marrow-derived cells with differentiated cells can occur spontaneously in vivo and acquire the phenotype of the recipient cells. In this study, we investigated the possibility of producing chromaffin-like cells by fusing human bone marrow-derived mesenchymal stem cells (MeSCs) with post-mitotic porcine CCs in vitro with the application of polyethylene glycol (PEG). METHODS AND RESULTS: Before cell-to-cell fusion was initiated, MeSCs and CCs were labeled by fluorescence dyes DiO (green) and Dil (red), respectively. The hybrid cells generated by PEG-mediated fusion expressed a DiO and Dil double-staining with estimated fusion efficiency at approximately 40% of the total cell population. Further immunocytochemical examination for tyrosine hydroxylase and methionine enkephalin (markers for CCs) demonstrated positive immuno-reactivity in these hybrid cells 2 weeks post-fusion. More interestingly, some of the hybrid cells showed bromodeoxyuridine (BrdU)-positive immunostaining in the nuclei. DISCUSSION: Our results show that these hybrid cells fused by CCs and MeSCs express some characteristics of the CC phenotype. A subpopulation of these hybrid cells are dividing cells with positive BrdU immunostaining, suggesting that a novel cellular production could be developed by a "reprogramming" mechanism through the application of targeted cell fusion strategies.
Guangbin Shi; Ke Ma; George D Pappas; Tingyu Qu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-04
Journal Detail:
Title:  Neurological research     Volume:  30     ISSN:  0161-6412     ISO Abbreviation:  Neurol. Res.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-25     Completed Date:  2008-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  217-22     Citation Subset:  IM    
The Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, 1601 West Taylor Street, Chicago, IL 60612, USA.
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MeSH Terms
Adrenal Medulla / cytology*
Bromodeoxyuridine / metabolism
Cell Count
Cell Differentiation / physiology
Cell Fusion / methods
Cells, Cultured
Chromaffin Cells / drug effects,  physiology*
Fluorescent Dyes / metabolism
Hybrid Cells / physiology*
Mesenchymal Stem Cells / drug effects,  physiology*
Polyethylene Glycols / pharmacology
Surface-Active Agents / pharmacology
Tyrosine 3-Monooxygenase / metabolism
Grant Support
Reg. No./Substance:
0/Fluorescent Dyes; 0/Polyethylene Glycols; 0/Surface-Active Agents; 59-14-3/Bromodeoxyuridine; EC 3-Monooxygenase

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