| Phenotypic characteristics of hybrid cells produced by cell fusion of porcine adrenal chromaffin cells with human mesenchymal stem cells: a preliminary study. | |
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MedLine Citation:
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PMID: 18252039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: Transplantation of adrenal chromaffin cells (CCs) that release endogenous opioid peptides and catecholamines produces significant antinociceptive effects in patients with terminal cancer pain. In clinical practice, however, obtaining a sufficient number of chromaffin cells may not be possible because of the limited availability of human adrenal tissue. Recent works have shown that fusion of bone marrow-derived cells with differentiated cells can occur spontaneously in vivo and acquire the phenotype of the recipient cells. In this study, we investigated the possibility of producing chromaffin-like cells by fusing human bone marrow-derived mesenchymal stem cells (MeSCs) with post-mitotic porcine CCs in vitro with the application of polyethylene glycol (PEG). METHODS AND RESULTS: Before cell-to-cell fusion was initiated, MeSCs and CCs were labeled by fluorescence dyes DiO (green) and Dil (red), respectively. The hybrid cells generated by PEG-mediated fusion expressed a DiO and Dil double-staining with estimated fusion efficiency at approximately 40% of the total cell population. Further immunocytochemical examination for tyrosine hydroxylase and methionine enkephalin (markers for CCs) demonstrated positive immuno-reactivity in these hybrid cells 2 weeks post-fusion. More interestingly, some of the hybrid cells showed bromodeoxyuridine (BrdU)-positive immunostaining in the nuclei. DISCUSSION: Our results show that these hybrid cells fused by CCs and MeSCs express some characteristics of the CC phenotype. A subpopulation of these hybrid cells are dividing cells with positive BrdU immunostaining, suggesting that a novel cellular production could be developed by a "reprogramming" mechanism through the application of targeted cell fusion strategies. |
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Authors:
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Guangbin Shi; Ke Ma; George D Pappas; Tingyu Qu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-02-04 |
Journal Detail:
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Title: Neurological research Volume: 30 ISSN: 0161-6412 ISO Abbreviation: Neurol. Res. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-25 Completed Date: 2008-08-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905298 Medline TA: Neurol Res Country: England |
Other Details:
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Languages: eng Pagination: 217-22 Citation Subset: IM |
Affiliation:
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The Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, 1601 West Taylor Street, Chicago, IL 60612, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Medulla
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cytology* Animals Bromodeoxyuridine / metabolism Cell Count Cell Differentiation / physiology Cell Fusion / methods Cells, Cultured Chromaffin Cells / drug effects, physiology* Fluorescent Dyes / metabolism Humans Hybrid Cells / physiology* Mesenchymal Stem Cells / drug effects, physiology* Phenotype* Polyethylene Glycols / pharmacology Surface-Active Agents / pharmacology Swine Tyrosine 3-Monooxygenase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01DA015511/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Polyethylene Glycols; 0/Surface-Active Agents; 59-14-3/Bromodeoxyuridine; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
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