Document Detail

Phenotypic characterization of a copA mutant of Neisseria gonorrhoeae identifies a link between copper and nitrosative stress.
MedLine Citation:
PMID:  22184419     Owner:  NLM     Status:  MEDLINE    
NGO0579 is annotated copA in the Neisseria gonorrhoeae chromosome, suggesting that it encodes a cation-transporting ATPase specific for copper ions. Compared to wild-type cells, a copA mutant was more sensitive to killing by copper ions but not to other transition metals. The mutant also accumulated a greater amount of copper, consistent with the predicted role of CopA as a copper efflux pump. The copA mutant showed a reduced ability to invade and survive within human cervical epithelial cells, although its ability to form a biofilm on the surface of these cells was not significantly different from that of the wild type. In the presence of copper, the copA mutant exhibited increased sensitivity to killing by nitrite or nitric oxide. Therefore, we concluded that copper ion efflux catalyzed by CopA is linked to the nitrosative stress defense system of Neisseria gonorrhoeae. These observations suggest that copper may exert its effects as an antibacterial agent in the innate immune system via an interaction with reactive nitrogen species.
Karrera Y Djoko; Jessica A Franiek; Jennifer L Edwards; Megan L Falsetta; Stephen P Kidd; Adam J Potter; Nathan H Chen; Michael A Apicella; Michael P Jennings; Alastair G McEwan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-19
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-16     Completed Date:  2012-04-09     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1065-71     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / genetics,  metabolism*
Cells, Cultured
Copper / metabolism,  toxicity*
Epithelial Cells / microbiology
Gene Deletion*
Microbial Viability / drug effects
Neisseria gonorrhoeae / drug effects,  genetics,  metabolism*
Nitric Oxide / toxicity
Nitrites / toxicity
Stress, Physiological*
Virulence Factors / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/CopA protein, Bacteria; 0/Nitrites; 0/Virulence Factors; 31C4KY9ESH/Nitric Oxide; 789U1901C5/Copper

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