Document Detail


Phenotypic changes and inflammatory cell distribution in the cornea during development of experimental immune-mediated blepharoconjunctivitis.
MedLine Citation:
PMID:  15295657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the morphological changes in the cornea during the development of experimental immune-mediated blepharoconjunctivitis (EC). METHODS: EC was induced in Brown Norway (BN) rats by active immunization with ovalbumin (OVA) emulsified in complete Freund's adjuvant and a subsequent challenge by OVA eyedrops. The corneas were analyzed immunohistochemically. RESULTS: Before the induction of EC, cells stained with OX6 (rat MHC class 2, RT1B), ED1 (tissue macrophages), ED2 (resident macrophages), CD4, or major basic protein were present in the peripheral corneal stroma. ED1- and OX6-stained cells were also observed in the central corneal stroma, and their number increased after the antigen challenge. Infiltration of cells stained with ED1, ED2, OX62 (dendritic cells), CD4, or CD3 (T cells) from the limbus to the peripheral corneal stroma started 6 h after the antigen challenge. Expression of MHC class 2 molecules was induced on the corneal epithelium by the antigen challenge. CONCLUSIONS: The present study demonstrates for the first time the phenotypic changes and distribution of inflammatory cells in the cornea during the development of EC.
Authors:
Akemi Ozaki; Waka Ishida; Kazuyo Fukata; Atsuki Fukushima; Hisayuki Ueno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of ophthalmology     Volume:  48     ISSN:  0021-5155     ISO Abbreviation:  Jpn. J. Ophthalmol.     Publication Date:    2004 Jul-Aug
Date Detail:
Created Date:  2004-08-05     Completed Date:  2004-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0044652     Medline TA:  Jpn J Ophthalmol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  333-9     Citation Subset:  IM    
Copyright Information:
Copyright Japanese Ophthalmological Society 2004
Affiliation:
Laboratory of Immunology, Department of Ophthalmology, Kochi Medical School, Nankoku, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / metabolism
Blepharitis / immunology,  pathology*
Conjunctivitis, Allergic / immunology,  pathology*
Cornea / pathology*
Disease Models, Animal
Eosinophils / pathology*
Immunoenzyme Techniques
Macrophages / pathology*
Male
Ovalbumin / immunology
Phenotype
Rats
Rats, Inbred BN
T-Lymphocytes / pathology*
Chemical
Reg. No./Substance:
0/Antigens, CD; 9006-59-1/Ovalbumin

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