Document Detail


Phenotypic changes in the Japanese encephalitis virus after one passage in Neuro-2a cells: generation of attenuated strains of the virus.
MedLine Citation:
PMID:  18045754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Live attenuated strains of the Japanese encephalitis (JE) virus are known to form in various cultured cells. In this study, we selected attenuated JE virus variants by passing the parent virus strains once through Neuro-2a cells, showing that the selection intensity ranged 2-1000-fold after infection for 3d. The selection of attenuated variants appeared in association with mutations on the envelope (E) glycoprotein. This is likely determined by the differential binding abilities of specific E proteins with highly sulfated glycosaminoglycans on the cell surface. A plaque-purification method showed that Neuro-2a-selected variants were usually less neurovirulent, leading to a longer survival time of intracerebrally inoculated mice. Specific selected variants (mostly with the small-plaque phenotype) were shown to be more efficient at replication in Vero cells and less virulent, particularly those with substitution of the E-138 (Glu-->Lys) residue. As a result, most, if not all, low-virulent variants were generated in a relatively short time, all which have the potential to be live-attenuated vaccine candidates of the JE virus.
Authors:
Shyan-Song Chiou; Wei-June Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-09
Journal Detail:
Title:  Vaccine     Volume:  26     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-02-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  15-23     Citation Subset:  IM    
Affiliation:
Graduate Institute of Veterinary Public Health, National Chung Hsing University, Taichung 40227, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / virology
Cell Line, Tumor
Cercopithecus aethiops
Encephalitis Virus, Japanese / drug effects,  growth & development*,  pathogenicity
Heparin / pharmacology
Japanese Encephalitis Vaccines*
Mice
Mice, Inbred ICR
Neuroblastoma / virology
Phenotype
Vaccines, Attenuated
Vero Cells
Virulence
Chemical
Reg. No./Substance:
0/Japanese Encephalitis Vaccines; 0/Vaccines, Attenuated; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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