Document Detail


Phenotypic analysis of human glioma cells expressing the MMAC1 tumor suppressor phosphatase.
MedLine Citation:
PMID:  10022807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MMAC1, also known as PTEN or TEP-1, was recently identified as a gene commonly mutated in a variety of human neoplasias. Sequence analysis revealed that MMAC1 harbored sequences similar to those found in several protein phosphatases. Subsequent studies demonstrated that MMAC1 possessed in vitro enzymatic activity similar to that exhibited by dual specificity phosphatases. To characterize the potential cellular functions of MMAC1, we expressed wild-type and several mutant variants of MMAC1 in the human glioma cell line, U373, that lacks endogenous expression. While expression of wild-type MMAC1 in these cells significantly reduced their growth rate and saturation density, expression of enzymatically inactive MMAC1 significantly enhanced growth in soft agar. Our observations indicate that while wild-type MMAC1 exhibits activities compatible with its proposed role as a tumor suppressor, cellular expression of MMAC1 containing mutations in the catalytic domain may yield protein products that enhance transformation characteristics.
Authors:
A M Morimoto; A E Berson; G H Fujii; D H Teng; S V Tavtigian; R Bookstein; P A Steck; J B Bolen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  18     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-03     Completed Date:  1999-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1261-6     Citation Subset:  IM    
Affiliation:
Department of Cell Signaling, DNAX Research Institute, Palo Alto, California 94304, USA.
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MeSH Terms
Descriptor/Qualifier:
Catalytic Domain / genetics
Cell Adhesion
Cell Division
Cell Transformation, Neoplastic
Genes, Tumor Suppressor*
Glioma / enzymology,  genetics*
Humans
Mutation
PTEN Phosphohydrolase
Phenotype
Phosphoric Monoester Hydrolases / biosynthesis*,  genetics
Recombinant Proteins / biosynthesis
Tumor Cells, Cultured
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Tumor Suppressor Proteins; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase

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