| Phenotypic Analysis of Neurospora crassa Gene Deletion Strains. | |
| | |
MedLine Citation:
|
PMID: 21590422 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Phenotypic analysis of Neurospora crassa knockout (KO) mutants was used as a vehicle to introduce students to laboratory research. The availability of gene deletion strains was the impetus for the development of a program designed to introduce beginning science students to basic microbiology, genetics, microscopy and beginning bioinformatics. The goal was to provide a research experience, acquire laboratory skills and phenotype hundreds of KO mutants. The data provided by the students was used to build a phenotype database at the Broad Institute at Harvard/MIT for the fungal scientific community. Each mutant analysis consists of five assays that examine growth and morphology, asexual and sexual development using wild-type (parental) strains as a reference. This information indicates how loss of each gene impacts these basic and important processes. |
| | |
Authors:
|
Gloria E Turner |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 722 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
|
Created Date: 2011-05-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 191-8 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA, turner@chem.ucla.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High-Throughput Production of Gene Replacement Mutants in Neurospora crassa.
Next Document: Efficient Approaches for Generating GFP Fusion and Epitope-Tagging Constructs in Filamentous Fungi.