Document Detail


Phenotype analysis of mice deficient in the peptide transporter PEPT2 in response to alterations in dietary protein intake.
MedLine Citation:
PMID:  16586099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The peptide transporter PEPT2 mediates cellular uptake of di- and tripeptides driven by an inwardly directed electrochemical proton gradient. In mammals PEPT2 is found in a variety of organs such as kidney, lung, brain, enteric nervous system, and mammary gland. Highest expression levels are observed in renal proximal tubules where PEPT2 contributes to reabsorption of filtered di- and tripeptides. To assess the physiological importance of the transporter in overall metabolism, we have generated a Pept2-/- mouse line that lacks a functional PEPT2 protein. Here we present data on body weight, organ weights, and blood pressure. Mice were then fed diets containing either 10, 20, or 30% (w/w) protein, and food and water intake rates as well as plasma and urine parameters were determined. In spite of PEPT2 expression in a variety of tissues, only subtle phenotypic changes were observed. Male PEPT2 null mice displayed lower bodyweight and lower relative heart weight, whereas, relative kidney weight was lower in female Pept2-/- mice. No differences were found in blood pressure. When fed diets with different protein contents, Pept2-/- mice adapted food intake to dietary protein content with higher consumption rates on low protein and reduced food intake rates on the high protein diet.
Authors:
Isabelle M Frey; Isabel Rubio-Aliaga; Martina Klempt; Eckhard Wolf; Hannelore Daniel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-04
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  452     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-22     Completed Date:  2007-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  300-6     Citation Subset:  IM    
Affiliation:
Molecular Nutrition Unit, Institute of Nutritional Sciences, Technical University of Munich, Am Forum 5, 85350, Freising-Weihenstephan, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Chemical Analysis
Blood Pressure
Body Weight
Dietary Proteins / metabolism*
Dipeptides / metabolism
Female
Male
Mice
Organ Size
Phenotype
Symporters / deficiency,  physiology*
Urinalysis
Chemical
Reg. No./Substance:
0/Dietary Proteins; 0/Dipeptides; 0/Symporters; 0/hydrogen-coupled oligopeptide transporter PepT2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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