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Phenolic Composition and Antioxidant Activity of Aqueous Infusions from Capparis spinosa L. and Crithmum maritimum L. Before and After Submission to a two - step In Vitro Digestion Model.
MedLine Citation:
PMID:  22017607     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This study investigated the phenolic composition and antioxidant activities of aqueous infusions from wild-grown caper (Capparis spinosa L.) and sea fennel (Crithmum maritimum L.) from Dalmatia region (Croatia) before and after their submission to an in vitro digestion process. HPLC/UV-Vis-DAD/ESI-MS analysis of caper infusion identified rutin, kaempferol 3-O-rutinoside and isorhamnetin 3-O-rutinoside as dominant flavonoids in the matrix together with a series of cinnamoyl-quinic acid derivatives, whilst in the sea fennel aqueous infusion chlorogenic acid (5-caffeoyl quinic acid), its isomers and higher derivatives were identified as almost the sole class of phenolics. Both infusions exhibited good and dose-dependent antioxidant activity before in vitro digestion by DPPH method, the -carotene bleaching method and copper-induced oxidation of human LDL. The amount of total phenolics (Folin-Ciocalteau assay) strongly decreased in digested samples (from 3.0 and 2.2% in caper and sea fennel infusions, respectively, to below 1.0%), as well as their antioxidant activity as measured by the three aforesaid methods. The results showed that the majority of phenolic compounds detected in both infusions are not stable under applied simulated gastro-intestinal conditions and that the stability of these secondary metabolites strongly depends on the nature of the corresponding matrix.
Authors:
Laura Siracusa; Tea Kulisic-Bilusic; Olivera Politeo; Ingolf Krause; Branka Dejanoviċ; Giuseppe Ruberto
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-22
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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