Document Detail


Phasor-FLIM analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells.
MedLine Citation:
PMID:  23019116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of the cellular distributions of coenzymes including NADH may aid in understanding a cells metabolic status. We altered serum concentration (0, 2, and 10%) to induce living myoblast cells to undergo the early stages of differentiation. Through microscopy and phasor-FLIM, we spatially mapped and identified variations in the distribution of free and bound NADH. Undifferentiated cells displayed abundant free NADH within the nucleus along with specific regions of more bound NADH. Complete serum starvation dramatically increased the fraction of bound NADH in the nucleus, indicating heightened requirement for transcriptional processes. In comparison, cells exposed to 2% serum exhibited intermediate free nuclear NADH fraction. Overall our results suggest an order of events in which a cell metabolic status alters significantly during the early stages of serum induced differentiation.
Authors:
Belinda K Wright; Laura M Andrews; Mark R Jones; Chiara Stringari; Michelle A Digman; Enrico Gratton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-28
Journal Detail:
Title:  Microscopy research and technique     Volume:  75     ISSN:  1097-0029     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-04-29     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1717-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / chemistry*
Coenzymes / analysis
Microscopy
Myoblasts / chemistry*
NAD / analysis*
Rats
Stem Cells / chemistry*
Grant Support
ID/Acronym/Agency:
P41 GM103540/GM/NIGMS NIH HHS; P41 GM103540/GM/NIGMS NIH HHS; P41 RR003155/RR/NCRR NIH HHS; P41-RR03155/RR/NCRR NIH HHS; P50 GM076516/GM/NIGMS NIH HHS; P50 GM076516/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Coenzymes; 0U46U6E8UK/NAD
Comments/Corrections

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