Document Detail


Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages.
MedLine Citation:
PMID:  16820455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the 16 typing phages fall into four groups based on their patterns of activity against spontaneous mutants. Screens of transposon and defined mutants indicate that the phage-bacterium interaction for one of these groups appears to involve the capsular polysaccharide (CPS), while two of the other three groups consist of flagellatropic phages. The expression of CPS and flagella is potentially phase variable in C. jejuni, and the implications of these findings for typing and intervention strategies are discussed.
Authors:
Chris Coward; Andrew J Grant; Craig Swift; Jennifer Philp; Rebecca Towler; Mohammad Heydarian; Jennifer A Frost; Duncan J Maskell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-05     Completed Date:  2006-09-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4638-47     Citation Subset:  IM    
Affiliation:
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Capsules / metabolism*
Bacterial Typing Techniques
Bacteriophage Typing*
Bacteriophages / physiology*
Campylobacter jejuni / classification,  genetics,  growth & development*,  virology*
DNA Transposable Elements
Flagella / metabolism*
Humans
Lysogeny
Mutation
Phenotype
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Bacterial Capsules; 0/DNA Transposable Elements
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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