Document Detail


Phase properties of liquid-crystalline Phosphatidylcholine/Phosphatidylethanolamine bilayers revealed by fluorescent probes.
MedLine Citation:
PMID:  10486148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mixing properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were examined in liquid-crystalline phase using fluorescent probes incorporated into lipid bilayers. The excimer to monomer (E/M) fluorescence ratio of 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (PPC) versus PPC concentration was higher for binary mixtures containing phosphatidylcholine (PC)/phosphatidylethanolamine (PE) (1:1) compared to PC matrix. When POPC was gradually replaced with POPE, the E/M ratio also increased suggesting the enhanced lateral mobility or the lateral enrichment of PPC into domains or both. Evidences for the PE-induced domain formation were further provided by resonance energy transfer between 2-(4, 4-difluoro-5-methyl-4-boro-3a, 4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero- 3-phospho choline and PPC, which was enhanced as a function of PE concentration, and by the polarization of 1,6-diphenyl-1,3, 5-hexatriene. In addition, PE reduced free volume and polarity of lipid bilayers as measured by the emission fluorescence of 1,2-bis PPC and 6-lauroyl-2-dimethylaminonaphthalene. When POPE analogs with a methylated head group instead of normal POPE were used, the diminished effect on the domain formation was shown in the order N-methyl PE > N,N-dimethyl PE. The results suggest that the mixing properties of POPE and POPC are not random but that lipid domains of phospholipids are formed.
Authors:
T Ahn; C H Yun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  369     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-11-03     Completed Date:  1999-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  288-94     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, 305-701, Korea.
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MeSH Terms
Descriptor/Qualifier:
2-Naphthylamine / analogs & derivatives
4-Chloro-7-nitrobenzofurazan / analogs & derivatives
Energy Transfer
Fluorescent Dyes
Laurates
Lipid Bilayers / chemistry*
Molecular Probes
Phosphatidylcholines / chemistry*
Phosphatidylethanolamines / chemistry*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/1,2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diazaindacene-3-dodecanoyl)-sn-glycero-3-phosphocholine; 0/1-acyl-2-(12-((7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl)phosphatidylcholine; 0/Fluorescent Dyes; 0/Laurates; 0/Lipid Bilayers; 0/Molecular Probes; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 10015-88-0/1-palmitoyl-2-oleoylphosphatidylethanolamine; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 74515-25-6/laurdan; 91-59-8/2-Naphthylamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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