| Phase properties of liquid-crystalline Phosphatidylcholine/Phosphatidylethanolamine bilayers revealed by fluorescent probes. | |
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MedLine Citation:
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PMID: 10486148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mixing properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were examined in liquid-crystalline phase using fluorescent probes incorporated into lipid bilayers. The excimer to monomer (E/M) fluorescence ratio of 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (PPC) versus PPC concentration was higher for binary mixtures containing phosphatidylcholine (PC)/phosphatidylethanolamine (PE) (1:1) compared to PC matrix. When POPC was gradually replaced with POPE, the E/M ratio also increased suggesting the enhanced lateral mobility or the lateral enrichment of PPC into domains or both. Evidences for the PE-induced domain formation were further provided by resonance energy transfer between 2-(4, 4-difluoro-5-methyl-4-boro-3a, 4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero- 3-phospho choline and PPC, which was enhanced as a function of PE concentration, and by the polarization of 1,6-diphenyl-1,3, 5-hexatriene. In addition, PE reduced free volume and polarity of lipid bilayers as measured by the emission fluorescence of 1,2-bis PPC and 6-lauroyl-2-dimethylaminonaphthalene. When POPE analogs with a methylated head group instead of normal POPE were used, the diminished effect on the domain formation was shown in the order N-methyl PE > N,N-dimethyl PE. The results suggest that the mixing properties of POPE and POPC are not random but that lipid domains of phospholipids are formed. |
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Authors:
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T Ahn; C H Yun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 369 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-11-03 Completed Date: 1999-11-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 288-94 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, 305-701, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Naphthylamine
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analogs & derivatives 4-Chloro-7-nitrobenzofurazan / analogs & derivatives Energy Transfer Fluorescent Dyes Laurates Lipid Bilayers / chemistry* Molecular Probes Phosphatidylcholines / chemistry* Phosphatidylethanolamines / chemistry* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/1,2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diazaindacene-3-dodecanoyl)-sn-glycero-3-phosphocholine; 0/1-acyl-2-(12-((7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl)phosphatidylcholine; 0/Fluorescent Dyes; 0/Laurates; 0/Lipid Bilayers; 0/Molecular Probes; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 10015-88-0/1-palmitoyl-2-oleoylphosphatidylethanolamine; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 74515-25-6/laurdan; 91-59-8/2-Naphthylamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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