| Phase-dependent effects of stimuli locked to oscillatory activity in cultured cortical networks. | |
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MedLine Citation:
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PMID: 20513388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The archetypal activity pattern in cultures of dissociated neurons is spontaneous network-wide bursting. Bursts may interfere with controlled activation of synaptic plasticity, but can be suppressed by the application of stimuli at a sufficient rate. We sinusoidally modulated (4 Hz) the pulse rate of random background stimulation (RBS) and found that cultures were more active, burst less frequently, and expressed oscillatory activity. Next, we studied the effect of phase-locked tetani (four pulses, 200 s(-1)) on network activity. Tetani were applied to one electrode at the peak or trough of mRBS stimulation. We found that when tetani were applied at the peak of modulated RBS (mRBS), a significant potentiation of poststimulus histograms (PSTHs) occurred. Conversely, tetani applied at the trough resulted in a small but insignificant depression of PSTHs. In addition to PSTHs, electrode-specific firing rate profiles within spontaneous bursts before and after mRBS were analyzed. Here, significant changes in firing rate profiles were found only for stimulation at the peak of mRBS. Our study shows that rhythmic activity in culture is possible, and that the network responds differentially to strong stimuli depending on the phase at which they are delivered. This suggests that plasticity mechanisms may be differentially accessible in an oscillatory state. |
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Authors:
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Jan Stegenga; Joost le Feber; Enrico Marani; Wim L C Rutten |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biophysical journal Volume: 98 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-01 Completed Date: 2010-09-02 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 2452-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Biomedical Signals and Systems Group, Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. j.stegenga@utwente.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials Animals Cells, Cultured Cerebral Cortex / physiology* Electric Stimulation Microelectrodes Neural Pathways / physiology Neuronal Plasticity / physiology* Periodicity Rats Rats, Wistar Time Factors |
| Comments/Corrections | |
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