Document Detail

Pharmacologically active microcarriers: a tool for cell therapy.
MedLine Citation:
PMID:  15621263     Owner:  NLM     Status:  MEDLINE    
To overcome certain problems encountered in cell therapy, particularly cell survival, lack of cell differentiation and integration in the host tissue, we developed pharmacologically active microcarriers (PAM). These biodegradable particles made with poly(D,L-lactic-co-glycolic acid) (PLGA) and coated with adhesion molecules may serve as a support for cell culture and may be used as cell carriers presenting a controlled delivery of active protein. They can thus support the survival and differentiation of the transported cells as well as their microenvironment. To develop this tool, nerve growth factor (NGF)-releasing PAM, conveying PC12 cells, were produced and characterized. Indeed, these cells have the ability to differentiate into sympathetic-like neurons after adhering to a substrate, in the presence of NGF, and can then release large amounts of dopamine. Certain parameters such as the size of the microcarriers, the conditions enabling the coating of the microparticles and the subsequent adhesion of cells were thus studied to produce optimized PAM.
V M Tatard; M C Venier-Julienne; P Saulnier; E Prechter; J P Benoit; P Menei; C N Montero-Menei
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2004-12-28     Completed Date:  2005-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3727-37     Citation Subset:  IM    
INSERM U 646, Laboratoire d'ingénierie de la Vectorisation Particulaire, 10 rue André Boquel, 49100 Angers, France.
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MeSH Terms
Cell Adhesion / drug effects
Cell Culture Techniques / methods*
Cell Differentiation / drug effects,  physiology
Cell Survival / drug effects,  physiology
Cell Transplantation / methods*
Coated Materials, Biocompatible / administration & dosage,  chemistry
Drug Carriers / chemistry*
Lactic Acid / chemistry*
Materials Testing
Nerve Growth Factor / administration & dosage*
Neurons / cytology*,  drug effects,  physiology*
PC12 Cells
Polyglycolic Acid / chemistry*
Polymers / chemistry*
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Drug Carriers; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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