Document Detail


Pharmacological mechanism responsible for the Atractylodes japonica-induced distal colonic contraction in rats.
MedLine Citation:
PMID:  20851585     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND AND AIM: Atractylodes japonica Koidz (Compositae) has been commonly used to treat the gastrointestinal (GI) disorders in Korean traditional medicine, but its pharmacological roles in the regulation of GI motility have not been clarified yet.
METHODS: Atractylodes japonica was sequentially partitioned with MeOH, n-hexane, CHCl(3), EtOAc and n-BuOH saturated with H(2)O, and the effects of Atractylodes japonica extracts on the spontaneous contractility of GI muscle strips prepared from rats were measured.
RESULTS: Among five different fractionations, EtOAc extracts of Atractylodes japonica (AJEA) dose-dependently increased the low frequency contraction of distal colon longitudinal muscles (DCLM), and the ED(50) values were revealed to be 1.71×10(-9)g/ml. Among GI tracts, a prominent contractile response to AJEA was observed only in the DCLM. The contractile patterns produced by AJEA remarkably differed from those caused by acetylcholine and 5-HT. 4-DAMP and methoctramine at 0.5μM significantly blocked the AJEA (1.0μg/ml)-induced contraction of DCLM, but ondansetron, GR113808 and methysergide at 1.0μM in combination did not change the AJEA-induced DCLM contractions. Acetylethylcholine mustard (5.0μM) significantly diminished the AJEA-induced DCLM contractions, whereas p-chlorophenyl alanine (1.0μM) did not affect the stimulatory effects of AJEA on the DCLM contractions.
CONCLUSION: The present results suggest that AJEA may specifically act on the DCLM among GI smooth muscles, and AJEA-induced DCLM contraction is likely mediated, at least, by activation of ChAT and acetylcholinergic muscarinic receptors.
Authors:
Keun Han Choi; Seung Il Jeong; Jun Ho Lee; Byung Soon Hwang; Sang Jun Kim; Seoul Lee; Bong Kyu Choi; Kyu Yong Jung
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Publication Detail:
Type:  Journal Article     Date:  2010-09-20
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  18     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  408-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Pharmacology, Wonkwang University School of Medicine, 344-2 Shinyong-dong, Iksan, Jeonbuk 570-749, Republic of Korea.
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