Document Detail


Pharmacological manipulation of blood and lymphatic vascularization in ex vivo-cultured mouse embryos.
MedLine Citation:
PMID:  23060242     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formation of new blood and lymphatic vessels is involved in many physiological and pathological processes, including organ and tumor growth, cancer cell metastasis, fluid drainage and lymphedema. Therefore, the ability to manipulate vascularization in a mammalian system is of particular interest to researchers. Here we describe a method for pharmacological manipulation of de novo and sprouting blood and lymphatic vascular development in ex vivo-cultured mouse embryos. The described protocol can also be used to evaluate the properties of pharmacological agents in growing mammalian tissues and to manipulate other developmental processes. The whole procedure, from embryo isolation to image quantification, takes 3-5 d, depending on the analysis and age of the embryos.
Authors:
Martin Zeeb; Jennifer Axnick; Lara Planas-Paz; Thorsten Hartmann; Boris Strilic; Eckhard Lammert
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-11
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-12     Completed Date:  2013-03-19     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1970-82     Citation Subset:  IM    
Affiliation:
Institute of Metabolic Physiology, Heinrich-Heine University Duesseldorf, Duesseldorf, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryo Culture Techniques*
Embryo, Mammalian / blood supply,  drug effects*
Lymphatic System / blood supply,  drug effects*
Mice
Mice, Inbred C57BL
Neovascularization, Physiologic / drug effects*
Vascular Endothelial Growth Factor Receptor-2 / antagonists & inhibitors
Chemical
Reg. No./Substance:
EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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