Document Detail


Pharmacological and functional evidence for extracellular 3,4-dihydroxyphenylacetic acid as an index of metabolic activity of the adrenergic neurons: an in vivo voltammetry study in the rat rostral ventrolateral medulla.
MedLine Citation:
PMID:  2133351     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Catecholamine metabolism was studied in vivo in the C1 adrenergic area of the rostral ventrolateral medulla oblongata in rats, using differential normal pulse voltammetry coupled with an activated carbon fiber microelectrode. Pharmacological evidence indicates that 3,4-dihydroxyphenylacetic acid, the major dopamine metabolite, is responsible for the electrochemical signal appearance in the C1 group, and that it reflects the catecholamine synthesis rate, as previously reported in the locus coeruleus. Indeed, 3,4-dihydroxyphenylacetic acid was estimated to be formed from 77% of the intracellular dopamine, since its synthesis was increased by only 23%, after blockade of the dopamine-beta-hydroxylase activity. Neuronal activation by retrograde electrical stimulation increased the electrochemical signal, as well as hemorrhage and hypotension, suggesting that the level of extracellular 3,4-dihydroxyphenylacetic acid is a good biochemical index of the C1 adrenergic cellular activity in baseline conditions and during cellular activation.
Authors:
J Y Gillon; F Richard; L Quintin; J F Pujol; B Renaud
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  37     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  1990  
Date Detail:
Created Date:  1992-03-10     Completed Date:  1992-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  421-30     Citation Subset:  IM    
Affiliation:
Laboratoire de Neuropharmacologie, Faculté de Pharmacie, UMR 105, CNRS, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / metabolism*
Animals
Biological Markers
Blood Pressure / drug effects,  physiology
Catecholamines / metabolism,  physiology
Dopamine beta-Hydroxylase / antagonists & inhibitors
Electric Stimulation
Electrochemistry
Electrophysiology
Extracellular Space / drug effects,  metabolism
Hemorrhage / physiopathology
Male
Medulla Oblongata / cytology,  metabolism*
Methyltyrosines / pharmacology
Neurons / drug effects,  metabolism*
Pargyline / pharmacology
Rats
Rats, Inbred Strains
Sympathetic Nervous System / cytology,  drug effects,  metabolism*
alpha-Methyltyrosine
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Catecholamines; 0/Methyltyrosines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 555-57-7/Pargyline; 658-48-0/alpha-Methyltyrosine; EC 1.14.17.1/Dopamine beta-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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