Document Detail


Pharmacological evidence that different alpha 1 adrenoceptor subtypes mediate contraction in rabbit prostate and hypogastric artery.
MedLine Citation:
PMID:  8931767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The alpha 1 adrenoceptor subtypes mediating contraction of rabbit prostate and hypogastric artery were pharmacologically characterized using an isolated organ bath technique. The prostate had the same sensitivity to the contractile action of methoxamine and phenylephrine, whereas the hypogastric artery was five times less sensitive to the action of methoxamine in comparison with phenylephrine. Clonidine elicited contraction in the hypogastric artery but not in the prostate. BMY7378 was about 70-fold more potent to antagonize the phenylephrine-induced contraction in the hypogastric artery (pA2 8.14) than in the prostate (pA2 6.28), and 5-methyl-urapidil was about three-fold more potent on prostrate than on hypogastric artery. The potency of different alpha 1-adrenoceptor antagonists tested in the rabbit prostate was significantly correlated with their binding affinity for the expressed recombinant alpha 1A-, but not alpha 1B- or alpha 1D-, adrenoceptor subtype, whereas, the potency of the alpha 1-adrenoceptor antagonists tested in the rabbit hypogastric artery was better correlated with the defined alpha 1D-adrenoceptor. Chloroethylclonidine produced a 10-fold rightward shift in the phenylephrine concentration-response curve in the hypogastric artery but only had a weak effect in the prostate. The results indicate that significant heterogeneity exists among alpha1-adrenoceptor in the rabbit hypogastric artery (alpha 1D-adrenoceptor) and the prostate (alpha 1A-adrenoceptor).
Authors:
S Delaflotte; M Auguet; P E Chabrier
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  158     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-28     Completed Date:  1997-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  241-51     Citation Subset:  IM    
Affiliation:
Institut Henri Beaufour Research Labs., Les Ulis, France.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Agonists / pharmacology
Adrenergic alpha-Antagonists / pharmacology
Animals
Arteries / drug effects,  physiology*
Male
Prostate / drug effects,  physiology*
Rabbits
Receptors, Adrenergic, alpha-1 / classification*,  drug effects,  physiology*
Stomach / blood supply
Vasoconstriction / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Adrenergic alpha-Antagonists; 0/Receptors, Adrenergic, alpha-1; 0/adrenergic receptor alpha(1a); 0/adrenergic receptor alpha(1b); 0/adrenergic receptor alpha(1d)

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