Document Detail


Pharmacological analysis of responses to ATP in the isolated and perfused canine coronary artery.
MedLine Citation:
PMID:  9369346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vascular responses of the isolated and perfused canine coronary artery to adenosine 5'-triphosphate (ATP) were analyzed pharmacologically. At basal perfusion pressure, ATP induced a vasoconstriction followed by a vasodilation dose-dependently. The potency order for vasoconstriction was alpha,beta-methylene ATP > 2-methylthio ATP > UTP > ATP. That for vasodilation was ATP > 2-methylthio ATP > alpha,beta-methylene ATP >> UTP in the preparations precontracted by 20 mM KCl. Aminophylline inhibited the vasodilation induced by adenosine, but not that induced by ATP. Alpha,beta-methylene ATP and suramin inhibited the vasoconstriction induced by ATP. Reactive blue 2 inhibited the vasodilation induced by ATP, but not the vasoconstriction. Removal of the endothelium by saponin and L-N(G)-nitroarginine inhibited the vasodilation induced by ATP, but indomethacin did not. The results suggest that ATP induces vasoconstriction via P2X purinoceptors on the smooth muscle and vasodilation via P2Y purinoceptors on the endothelium through mainly the release of nitric oxide in the canine coronary artery, respectively.
Authors:
T Matsumoto; T Nakane; S Chiba
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  European journal of pharmacology     Volume:  334     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1998-01-22     Completed Date:  1998-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  173-80     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology*
Animals
Coronary Vessels / drug effects*,  physiology
Cyclooxygenase Inhibitors / pharmacology
Dogs
Endothelium, Vascular / physiology
Epoprostenol / antagonists & inhibitors
Female
Male
Muscle, Smooth, Vascular / drug effects*,  physiology
Nitric Oxide / antagonists & inhibitors
Nitric Oxide Synthase / antagonists & inhibitors
Perfusion
Receptors, Purinergic P1 / antagonists & inhibitors,  physiology
Receptors, Purinergic P2 / antagonists & inhibitors,  physiology
Vasoconstriction / drug effects*
Vasodilation / drug effects*
Chemical
Reg. No./Substance:
0/Cyclooxygenase Inhibitors; 0/Receptors, Purinergic P1; 0/Receptors, Purinergic P2; 10102-43-9/Nitric Oxide; 35121-78-9/Epoprostenol; 56-65-5/Adenosine Triphosphate; EC 1.14.13.39/Nitric Oxide Synthase

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