| Pharmacokinetics of laetispicine and its brain distribution in rats. | |
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MedLine Citation:
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PMID: 20821821 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Laetispicine, which is a novel amide alkaloid isolated from the stem of Piper laetispicum, has been proven to possess antidepressant and antinociceptive effects. This study examined the pharmacokinetic characteristics of laetispicine in plasma and brain distribution in rats by a simple sensitive HPLC-UV method. The separation was performed on a reverse-phase ODS column (250 mm x 4.6 mm, i.d., 5 microm) with a mobile phase composed of acetonitrile-water (75:25, v/v) as the mobile phase at a flow rate of 1.0 ml/min with UV detection at 259 nm. The calibration curve of laetispicine in rat plasma showed excellent linear behavior between 0.005-5.0 microg/ml (r2 = 0.9992), and between 0.02-0.5 microg/ml (r2 = 0.9952) in rat brain samples. The lower limit of quantification (LLOQ) was found to be 0.005 microg/ml in rat plasma and 0.02 microg/ml in rat brain samples. This HPLC assay was a precise and reliable method for the analysis of laetispicine in pharmacokinetic studies. Laetispicine was rapidly and extensively transported across the blood-brain barrier (BBB) and distributed into different brain regions. |
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Authors:
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Yong Wang; Hui Xie; Sheng-Li Pan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of Chinese medicine Volume: 38 ISSN: 0192-415X ISO Abbreviation: Am. J. Chin. Med. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-09-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7901431 Medline TA: Am J Chin Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 895-907 Citation Subset: IM |
Affiliation:
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School of Pharmacy, Fudan University, Shanghai, 201203, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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