Document Detail


Phagocytosis of mastitis isolates of Staphylococcus aureus and expression of type 5 capsular polysaccharide are influenced by growth in the presence of milk.
MedLine Citation:
PMID:  2229349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytosis by bovine polymorphonuclear granulocytes of seven capsular polysaccharide type 5 Staphylococcus aureus strains isolated from mastitis [corrected] was investigated by means of luminol-dependent chemiluminescence. Bacteria were grown on four different agar media (brain heart infusion, Columbia broth, modified staphylococcus medium 110, and skim milk) and were opsonized by normal bovine serum. When compared to growth on brain heart infusion agar, Columbia agar, and modified staphylococcus medium 110 agar, growth on skim milk agar rendered five of the strains more resistant to opsonization. The other two strains were resistant in all culture media used. Short periods of incubation in milk after growth on brain heart infusion agar did not augment resistance to phagocytosis, indicating that mere adsorption of milk components on bacteria was not responsible. The variability of the chemiluminescence response of polymorphonuclear leukocytes was pronounced among strains with each growth medium except milk. Growth on modified staphylococcus medium 110 and on milk agar favored the masking of teichoic acid, as shown by inagglutinability with rabbit antiserum. Interestingly, agglutination by a monoclonal antibody to capsular polysaccharide type 5 was optimal when bacteria were grown on skim milk agar. This suggests that capsular polysaccharide participated in the masking effect. These findings indicate that masking of the bacterial target of most of the naturally acquired opsonins present in normal bovine serum occurred when bacteria grew in the presence of milk, resulting in an increased resistance to phagocytosis by polymorphonuclear leukocytes.
Authors:
L Sutra; P Rainard; B Poutrel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  28     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-12-20     Completed Date:  1990-12-20     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2253-8     Citation Subset:  IM    
Affiliation:
Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, Nouzilly, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial
Bacterial Capsules*
Cattle
Culture Media
Female
Luminescent Measurements
Mastitis, Bovine / microbiology*
Milk / microbiology
Neutrophils / microbiology,  physiology
Opsonin Proteins
Phagocytosis*
Polysaccharides, Bacterial / immunology,  metabolism
Staphylococcal Infections / microbiology,  veterinary*
Staphylococcus aureus / growth & development,  immunology,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Bacterial Capsules; 0/Culture Media; 0/Opsonin Proteins; 0/Polysaccharides, Bacterial; 0/capsular polysaccharide, type 5, Staphylococcus aureus
Comments/Corrections
Erratum In:
J Clin Microbiol 1990 Dec;28(12):2853

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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