| Phagocytosis of candida albicans by lymphatic tumour cells in vitro. | |
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MedLine Citation:
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PMID: 12831164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experiments were carried out to investigate whether different lymphatic tumour cell lines have similar kinetic characteristics of phagocytosis of microorganisms. Six tumour cell lines were used. These were a human T-cell line (CEM), a mouse T-cell line (YAC-1), a human B-cell line (LAZ), and a human erythroleukemic tumour cells (K562), whereas 2 cell lines of professional phagocytosis were used as controls, a human macrophage cell line (THP1) and a mouse macrophage cell line (P388D1). Tumour cells were mixed with candida albicans at a ratio of 10:1 of candida to tumour cells and the percentage of tumour cells that had attached/phagocytosed candida was determined. After 4 h coculture with candida, tumour cells not of T-cell origin (LAZ and K562) showed moderate level of phagocytosis (28%), whereas tumour cells of T-cell origin (CEM and YAC-1) demonstrated low levels of phagocytosis (15%) as compared to macrophage cell lines (THP1 and P388D1) that showed maximum phagocytosis (64-78%). Acid phosphatase (AcPase) activity was increased by 33% during coculture of YAC-1 cells and yeast cells. In conclusion, the results suggest that lymphatic tumour cells of nonphagocytic origin acquire phagocytic properties during the course of malignancy, and digestion of phagocytosed yeast cells maybe related with AcPase activity, as well as that of other lysosomal enzymes. This phenomenon may represent one mechanism by which tumour cells downregulate immune surveillance. |
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Authors:
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Mamdooh Ghoneum; Iqbal Grewal; Jimmy Brown; Ryan Osborne; Hania Elembabi; Gus Gill |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Acta histochemica Volume: 105 ISSN: 0065-1281 ISO Abbreviation: Acta Histochem. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-06-30 Completed Date: 2004-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370320 Medline TA: Acta Histochem Country: Germany |
Other Details:
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Languages: eng Pagination: 127-33 Citation Subset: IM |
Affiliation:
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Charles R. Drew University of Medicine and Science, Department of Otolaryngology, Los Angeles, CA 90059, USA. mghoneum@ucla.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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metabolism Animals Candida albicans / physiology* Cell Line Cell Line, Tumor Coculture Techniques Humans K562 Cells Mice Phagocytosis / physiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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