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Phagocytic and oxidative burst activity of neutrophils in the Patients with Spinal Cord Injury.
MedLine Citation:
PMID:  23022452     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate phagocytic activity and neutrophil oxidative burst functions in patients with spinal cord injury (SCI) since alterations in neutrophil metabolic activity can be one of the causes of immune mechanism damage contributing to repeated bacterial infections. DESIGN: A controlled and cross-sectional study. SETTING: Departments of Physical Medicine & Rehabilitation and Immunology. PARTICIPANTS: A total of 34 patients with SCI and 28 healthy controls. INTERVENTIONS: Phagocytosis and oxidative burst in whole-blood neutrophils were assessed by flow cytometry. Percentage of phagocytizing cells after in vitro incubation with E. coli, phagocytic activity (mean intensity of fluorescence, MIF) and the percentage of neutrophil oxidative burst, and MIF value of production of reactive oxygen intermediates (ROIs) were analyzed. In addition, clinical assessment including the level of injury, ASIA scores, and functional status were carried out. MAIN OUTCOME MEASURES: Not Applicable. RESULTS: Although the percentage of E. coli phagocytizing neutrophils was not different between groups, the MIF value of absorbed E. coli was significantly lower in the patients with SCI vs controls (p<0.05). The MIF value of ROI production by neutrophils with both stimulator of phorbol 12-myristate 13-acetate (PMA) and E.Coli was significantly higher in patients with SCI (p<0.05). CONCLUSION: In patients with SCI, decreased phagocytic activity of neutrophils may be a result of a regulatory mechanism to minimize the deleterious effects of increased neutrophil burst activity.
Authors:
Selcen Kanyilmaz; Simin Hepguler; Funda C Atamaz; Nihal M Gokmen; Omur Ardeniz; Aytul Sin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Archives of physical medicine and rehabilitation     Volume:  -     ISSN:  1532-821X     ISO Abbreviation:  Arch Phys Med Rehabil     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985158R     Medline TA:  Arch Phys Med Rehabil     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Physical Medicine and Rehabilitation, Izmir, Turkey.
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