Document Detail


Phagocytic efficacy of macrophage-like cells as a function of cell cycle and Fcgamma receptors (FcgammaR) and complement receptor (CR)3 expression.
MedLine Citation:
PMID:  16879260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have shown that the efficiency of phagocytosis is a function of cell cycle and that phagocytosis promotes cell cycle progression. Because phagocytosis is dependent on cellular receptors we hypothesized that Fcgamma receptors (FcgammaR) and complement receptors (CR) expression varied with cell cycle. Consequently, we used centrifugal elutriation of macrophage-like cells, fluorescence activated cell sorting analysis and receptor staining to investigate expression of FcgammaR and CR as a function of cell cycle. We confirmed that FcgammaR expression on macrophage-like cells increased as the cells progressed from G1 to G2 phases. Moreover, CR3 expression varied as a function of cell cycle in a manner similar to FcgammaR. Correlation of receptor expression with cell size showed that FcgammaR and CR3 expression on macrophages was determined largely by cell size enlargement during the cell cycle. The efficacy of both Fc- and complement-mediated phagocytosis of live Cryptococcus neoformans (Cn) showed a biphasic pattern with the efficacy of phagocytosis decreasing when the cells approached the G1-S interface, which paralleled the changes in receptor surface expression when cells exited G1 phase. Live Cn cells were significantly more resistant to phagocytosis than dead cells at all stages of macrophage-like cell cycle. In contrast to live cells, the efficacy of phagocytosis of dead Cn decreased as surface receptor expression increased. Hence, the efficacy of phagocytosis in this system as function of cell cycle is not related to phagocytic receptor expression.
Authors:
Y Luo; E Cook; B C Fries; A Casadevall
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  145     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-01     Completed Date:  2006-09-21     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  380-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Line
Cell Size
Complement C3 / metabolism
Cryptococcus neoformans*
Flow Cytometry
Macrophage-1 Antigen / metabolism*
Macrophages, Peritoneal / cytology,  immunology*
Mice
Phagocytosis*
Receptors, Fc / metabolism*
Grant Support
ID/Acronym/Agency:
AI 033142/AI/NIAID NIH HHS; AI 033774/AI/NIAID NIH HHS; AI 052733/AI/NIAID NIH HHS; HL 059842/HL/NHLBI NIH HHS; R01 AI059681/AI/NIAID NIH HHS; R01 AI059681-05/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Complement C3; 0/Macrophage-1 Antigen; 0/Receptors, Fc
Comments/Corrections

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