| Phage library-screening: a powerful approach for generation of targeting-agents specific for normal pancreatic islet-cells and islet-cell carcinoma in vivo. | |
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MedLine Citation:
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PMID: 19958795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phage display technology is a powerful approach for the generation of peptides and antibodies that target specific organ- or tumor-structures. By applying this approach to rats in vivo or freshly isolated rat islets in vitro, we have recently reported the successful isolation of internalizing single-chain antibodies (SCA-antibodies), which are highly specific for the endocrine-cells of a pancreatic islet (either beta- or alpha-cells) both in rodents and in humans. Moreover, others have reported peptides targeting specifically the vascular endothelium of normal or pre-malignant islets or advanced islet-cell tumors. The features of these antibodies and peptides are compatible with a potential use for in vivo delivery of molecular cargos (e.g. imaging agents and therapeutics). Therefore, this article reviews the principles of phage display, provides an overview about agents either specific for the endocrine-cells or the vascular endothelium of islets, discusses methododical key elements for the generation of these ligands and highlights remaining questions and potential future perspectives. |
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Authors:
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S Ueberberg; S Schneider |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2009-12-01 |
Journal Detail:
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Title: Regulatory peptides Volume: 160 ISSN: 1873-1686 ISO Abbreviation: Regul. Pept. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100479 Medline TA: Regul Pept Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Medical Department 1, Division of Endocrinology and Metabolism, University Hospital Bergmannsheil, Ruhr-University of Bochum, 44789 Bochum, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / metabolism Bacteriophages / genetics* Blood Vessels / metabolism Cell Line, Tumor Drug Delivery Systems* Humans Islets of Langerhans / metabolism* Ligands Pancreatic Neoplasms Peptide Library* Peptides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Ligands; 0/Peptide Library; 0/Peptides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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