| Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGF. | |
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MedLine Citation:
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PMID: 22581629 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Vascular endothelial growth factor receptor 2 (VEGFR-2) plays a critical role in tumor angiogenesis. None therapeutic antibodies targeting VEGFR-2 are available in clinical use. Herein, we describe the screening of a new single-chain antibody fragment (scFv) targeting extracellular domain 3 of human VEGFR-2 (KDR3) from Griffin phage display scFv library. A comprehensive sequence analysis was performed to assign the framework and complementary-determining regions. The scFv exerted particular binding sites to KDR3 on Molecular Docking, and the binding affinity was further convinced by binding analysis both in quantitative ELISA and real-time kinetic determination by biosensors (K(D) = 40 nM).Finally, the scFv was revealed to inhibit VEGF-stimulated proliferation of human umbilical vein endothelial cells (HUVECs) (IC(50) = 5 nM) and to inhibit HUVEC migration significantly at 17 nM. Taken together, our results indicate that we have successfully isolated a scFv which differentially recognizes KDR3 and has potential clinical applications in the treatment of angiogenesis related diseases. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012. |
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Authors:
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Juan Zhang; Haixin Li; Xiuyun Wang; Haidi Qi; Xiaoniu Miao; Tao Zhang; Guosheng Chen; Min Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-11 |
Journal Detail:
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Title: Biotechnology progress Volume: - ISSN: 1520-6033 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 American Institute of Chemical Engineers (AIChE). |
Affiliation:
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State Key Laboratory of Natural Medicines, Department of Molecular Biology, China Pharmaceutical University, Nanjing 210009, China. juancpu@126.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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