Document Detail


Pesticide residue analysis in foodstuffs applying capillary gas chromatography with atomic emission detection state-of-the-art use of modified multimethod S19 of the Deutsche forschungsgemeinschaft and automated large-volume injection with programmed-temperature vaporization and solvent venting.
MedLine Citation:
PMID:  8938393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Atomic emission detection (AED) provides high element-specific detection of all compounds amenable to gas chromatography (GC). The heteroatoms nitrogen, chlorine, phosphorus, sulfur, bromine and fluorine, which are important elements in pesticide residue analysis, are of major interest. A main drawback of AED is its lower sensitivity with respect to other selective detection methods used in pesticide residue analysis such as electron-capture and nitrogen-phosphorus detection. This holds true especially for the important nitrogen trace. For this reason, more sensitive detection can be achieved by injection of larger volumes or higher concentrations of sample extracts, because matrix compounds were usually registered only in the carbon, hydrogen and oxygen traces. This paper focuses on recent developments from the authors' laboratory in order to demonstrate the feasibility of screening analyses with the identification of pesticide residues down to the 0.01 ppm concentration level in plant foodstuffs. This has been achieved by means of automated large volume injection with programmed-temperature vaporization and solvent venting as well as careful optimization of make-up and reactant gases with AED. Clean up follows the principle of multimethod S19 of the Deutsche Forschungsgemeinschaft in a reduced procedure. After elimination of lipids and waxes by gel permeation chromatography, extracts from 10 g of the food samples were concentrated to 200 microliters, of which 12.5 microliters were introduced into the GC-AED system. Two analyses were usually performed with the element traces of sulfur, phosphorus, nitrogen and carbon in the first run and chlorine and bromine in the second run. Fluorine and oxygen were not detected in any screening analyses. The method has proved to be of great value especially with "problem foodstuffs". The limits of detection were determined for 385 pesticides and are presented together with their retention data.
Authors:
H J Stan; M Linkerhägner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  750     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-15     Completed Date:  1997-01-15     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  369-90     Citation Subset:  IM    
Affiliation:
Technical University Berlin, Institute of Food Chemistry, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas / methods*
Food Analysis / methods*
Osmolar Concentration
Pesticide Residues / analysis*,  chemistry,  metabolism
Reproducibility of Results
Sensitivity and Specificity
Silicon Dioxide / chemistry
Solvents / chemistry
Temperature
Volatilization
Chemical
Reg. No./Substance:
0/Pesticide Residues; 0/Solvents; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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