| Pesticide Roundup provokes cell division dysfunction at the level of CDK1/cyclin B activation. | |
| | |
MedLine Citation:
|
PMID: 11896679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To assess human health risk from environmental chemicals, we have studied the effect on cell cycle regulation of the widely used glyphosate-containing pesticide Roundup. As a model system we have used sea urchin embryonic first divisions following fertilization, which are appropriate for the study of universal cell cycle regulation without interference with transcription. We show that 0.8% Roundup (containing 8 mM glyphosate) induces a delay in the kinetic of the first cell cleavage of sea urchin embryos. The delay is dependent on the concentration of Roundup. The delay in the cell cycle could be induced using increasing glyphosate concentrations (1-10 mM) in the presence of a subthreshold concentration of Roundup 0.2%, while glyphosate alone was ineffective, thus indicating synergy between glyphosate and Roundup formulation products. The effect of Roundup was not lethal and involved a delay in entry into M-phase of the cell cycle, as judged cytologically. Since CDK1/cyclin B regulates universally the M-phase of the cell cycle, we analyzed CDK1/cyclin B activation during the first division of early development. Roundup delayed the activation of CDK1/cyclin B in vivo. Roundup inhibited also the global protein synthetic rate without preventing the accumulation of cyclin B. In summary, Roundup affects cell cycle regulation by delaying activation of the CDK1/cyclin B complex, by synergic effect of glyphosate and formulation products. Considering the universality among species of the CDK1/cyclin B regulator, our results question the safety of glyphosate and Roundup on human health. |
| | |
Authors:
|
Julie Marc; Odile Mulner-Lorillon; Sandrine Boulben; Dorothée Hureau; Gaël Durand; Robert Bellé |
Related Documents
:
|
17877709 - The cell cycle of sulfolobus. 2768189 - Cell-cycle-dependent polar morphogenesis in caulobacter crescentus: roles of phospholip... 17426449 - Cell cycle dependent nucleolar localization of the coronavirus nucleocapsid protein. 9311989 - Dbf2, a cell cycle-regulated protein kinase, is physically and functionally associated ... 22359379 - Hydroxycinnamate conjugates as potential monolignol replacements: in vitro lignificat... 15087469 - Foxo1a can alter cell cycle progression by regulating the nuclear localization of p27ki... 11283949 - Structural abnormalities develop in the brain after ablation of the gene encoding nonmu... 8893739 - Differentiating neuroblastoma of pituitary gland: neuroblastic transformation of epithe... 8671339 - Stromal cell interaction and relevance to predecidual events and menstruation. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chemical research in toxicology Volume: 15 ISSN: 0893-228X ISO Abbreviation: Chem. Res. Toxicol. Publication Date: 2002 Mar |
Date Detail:
|
Created Date: 2002-03-18 Completed Date: 2002-07-16 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8807448 Medline TA: Chem Res Toxicol Country: United States |
Other Details:
|
Languages: eng Pagination: 326-31 Citation Subset: IM |
Affiliation:
|
Station Biologique de Roscoff, Université Pierre et Marie Curie (UFR 937), Centre National de la Recherche Scientifique (CNRS, UMR 7127), BP 74, 29682 Roscoff Cedex, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals CDC2 Protein Kinase / metabolism* Cell Cycle / drug effects Cell Division / drug effects Cyclin B / metabolism Embryo, Nonmammalian Enzyme Activation / drug effects Glycine / analogs & derivatives*, pharmacology*, toxicity Herbicides / pharmacology*, toxicity Ovum Sea Urchins Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Cyclin B; 0/Herbicides; 1071-83-6/glyphosate; 56-40-6/Glycine; EC 2.7.11.22/CDC2 Protein Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro inhibition of the enzymatic activity of tumor suppressor FHIT gene product by carcinogenic ...
Next Document: Microsomal activation of dibenzo[def,mno]chrysene (anthanthrene), a hexacyclic aromatic hydrocarbon ...